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Thromb Haemost 1973; 30(01): 138-147
DOI: 10.1055/s-0038-1649110
Original Article
Schattauer GmbH

The Filter Loop Technique as a Method of Measuring Platelet Aggregation in the Flowing Blood of the Rat; the Inhibitory Activity of 5-oxo-l-cyclopentene-l-heptanoic Acid (AY-16,804) on Platelet Aggregation[*]

Christopher R. Muirhead Ph.D.
1   Department of Pharmacology, Ay erst Research Laboratories, P. O. Box 6115, Montreal 101, Quebec, Canada
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Further Information

Publication History

Received for publication 06 February 1973

Accepted for publication 22 March 1973

Publication Date:
30 June 2018 (online)

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Summary

The filter loop technique which measures platelet aggregation in vivo in the flowing-blood of the rat was compared to the optical density technique of Born which is carried out in vitro with platelet rich plasma. Using these two experimental models the effect on platelet aggregation of three known inhibitors sulfinpyrazone, dipyridamole and prostaglandin E1, and a novel compound 5-oxo-l-cyclopentene-l-heptanoic acid (AY-16, 804) was determined.

The effects on platelet aggregation of the known inhibitors were consistent with information in the literature. Prostaglandin E1 was the most potent inhibitor in both techniques; sulfinpyrazone inhibited aggregation in both models but was less potent than prostaglandin E1. AY-16, 804 exhibited activity in vitro and in vivo similar to that of sulfinpyrazone. Dipyridamole did not inhibit platelet aggregation in vivo and did not inhibit aggregation in vitro in concentrations at which it remained soluble.

The filter loop technique is a suitable model for measuring platelet aggregation in the flowing blood of the rat. It is a relatively simple method of determining aggregation and easily adapted to other species.

* Presented in part at the 56th Annual Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N. J., April 9-14, 1972.


 

  • References

  • 1 Bagli J. F, Bogri T, Deghenghi R, and Wiesner K. 1966; Prostaglandins I. Total synthesis of 9β,15ξ-Dihydroxy prost-13-enoic acid. Tetrahedron Letters 5: 465.
    PubMedGoogle Scholar
  • 2 Born G. V. R. 1962; Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (London) 194: 927.
    PubMedGoogle Scholar
  • 3 Born G. V. R, and Cross M. J. 1963; Effect of adenosine diphosphate on the concentration of platelets in circulating blood. Nature (London) 197: 974.
    CrossrefPubMedGoogle Scholar
  • 4 Brinkhous K. M, Leroy E. C, Cornell W. P, Brown R. C, Hazlehurst J. L, and Vennart G. P. 1958; Macroscopic studies on platelet agglutination; nature of thrombocyte agglutinating activity in plasma. Proceedings of the Society for Experimental Biology and Medicine 98: 379.
    PubMedGoogle Scholar
  • 5 Bunag R. D, Douglas C. R, Imai S, and Berne R. M. 1964; Influence of a pyrimidopyrimidine derivative on deamination of adenosine by blood. Circulation Research 15: 83.
    CrossrefPubMedGoogle Scholar
  • 6 Chandler A. B. 1958; In vitro thrombotic coagulation of the blood. A method for producing a thrombus. Laboratory Investigation 7: 110.
    PubMedGoogle Scholar
  • 7 Davey M. G, and Lander H. 1964; Effect of adenosine diphosphate on circulating platelets in man. Nature (London) 201: 1037.
    PubMedGoogle Scholar
  • 8 Emmons P. R, Harrison M. J. G, Honour A. J, and Mitchell J. R. A. 1965; Effect of pyrimidopyrimidine derivative on thrombus formation in the rabbit. Nature (London) 208: 255.
    CrossrefPubMedGoogle Scholar
  • 9 Emmons P. R, Hampton J. R, Honour M. J. G, and Mitchell J. R. A. 1967; Effect of prostaglandin E1 on platelet behavior in vitro and in vivo. British Medical Journal 2: 468.
    CrossrefPubMedGoogle Scholar
  • 10 Heslinga L. M, Vangoekom M, and Vandorp D. A. 1968; Preparation and diastereoisome-rism of four substituted epoxycyclopentanones. Recueil des Travaux Chimiques des Pay Bas 87: 1421.
    PubMedGoogle Scholar
  • 11 Honour A. J, and Mitchell J. R. A. 1964; Platelet clumping in injured vessels. British Journal of Experimental Pathology 45: 75.
    PubMedGoogle Scholar
  • 12 Hornstra G. 1970; a A method for the determination of thrombocyte aggregation in circulating rat blood. Experientia 26: 111.
    CrossrefPubMedGoogle Scholar
  • 13 Hornstra G. 1970; b Method to determine the degree of ADP-induced platelet aggregation in circulating rat blood (“filter loop technique”). British Journal of Haematology 19: 321.
    CrossrefPubMedGoogle Scholar
  • 14 Kloeze J. 1967 Influence of prostaglandins on platelet adhesiveness and platelet aggregation. In: Bergstrom S, and Samuelsson B. (eds.) Interscience. Proceedings of the Second Nobel Symposium, Stockholm, June 1966.
    PubMedGoogle Scholar
  • 15 Mustard J. F, Rowsell H. C, Lutz F, Hegardt B, and Murphy E. A. 1968; The effect of adenine nucleotides on thrombus formation, platelet count and blood coagulation. Experimental and Molecular Pathology 5: 43.
    PubMedGoogle Scholar
  • 16 O’Brien J. R. 1962; Platelet aggregation II. Some results from a new method of study. Journal of Clinical Pathology (London) 15: 452.
    CrossrefPubMedGoogle Scholar
  • 17 Sullivan J. M, Harken D. E, and Gorlin R. 1968; Pharmacologic control of thromboembolic complications of cardiac valve replacement. New England Journal of Medicine 279: 576.
    CrossrefPubMedGoogle Scholar
  • 18 Swank R. L, Roth J. G, and Jansen J. 1964; Screen filtration pressure method and adhesiveness and aggregation of blood cells. Journal of Applied Physiology 19: 340.
    CrossrefPubMedGoogle Scholar
  • 19 Weily H. S, and Genton E. 1970; Altered platelet function in patients with prosthetic mitral valves: Effects of sulfinpyrazone therapy. American Journal of Cardiology 26: 668.
    PubMedGoogle Scholar