Observations on the Hydrolysis of p-Nitrophenyl Acylates by Purified Bovine Thrombin

Roger L. Lundblad

doi:10.1055/s-0038-1649071

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(02): 248-254
DOI: 10.1055/s-0038-1649071

Original Article

Schattauer GmbH

Observations on the Hydrolysis of p-Nitrophenyl Acylates by Purified Bovine Thrombin

Roger L. Lundblad

¹Dental Research Center and the Departments of Pathology and Biochemistry, University of North Carolina, Chapel Hill, North Carolina 27514 USA

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Abstract

Summary

The ability of purified bovine thrombin to catalyze the hydrolysis of p-nitrophenyl acetate and p-nitrophenyl butyrate has been studied. The butyrate derivative appears to be hydrolyzed more rapidly than the acetate derivative. An examination of the time course of hydrolysis of these compounds by thrombin shows an initial ‘‘burst” reaction followed by a slower ‘‘steady-state” reaction. The hydrolysis of p-nitrophenyl acylates by thrombin is inhibited by the presence of benzamidine as well as by prior reaction of thrombin with phenylmethylsulfonyl fluoride. It is suggested that bovine thrombin catalyzes the hydrolysis of p-nitrophenyl acylates and that the reaction proceeds via the formation of an acyl-enzyme intermediate.

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References

References
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