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DOI: 10.1055/s-0038-1649069
Preparation of Modified Bovine Factor VIII with Enhanced Biological Activity Using Insoluble-trypsin Columns
Publication History
Received for publication
16 May 1973
Accepted for publication
03 August 1973
Publication Date:
30 June 2018 (online)
Summary
Crude bovine Factor VIII preparations have been modified by passage over a column of trypsin-agarose. Factor VIII prepared without an early adsorption step utilizing either barium sulfate or aluminum hydroxide gel generated significant Factor Xa activity by passage over such a column and was unsuitable for further characterization. Factor VIII prepared using adsorption with barium sulfate contained no Factor Xa activity after passage over trypsin-agarose. This trypsin-modified material showed a decreased molecular size as judged by behavior on gel filtration and an increased rate of reaction with bovine Factor IXa.
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References
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