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Thromb Haemost 1972; 28(02): 317-324
DOI: 10.1055/s-0038-1649013
Original Article
Schattauer GmbH

Gel Filtration Properties of Factors II, VII, IX, IXa, and X

Bjarne Østerud   *
1   Departments of Medicine and Biochemistry, University of Southern California School of Medicine, Los Angeles, California, 90033
,
Sandra Schiffman
1   Departments of Medicine and Biochemistry, University of Southern California School of Medicine, Los Angeles, California, 90033
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Publikationsdatum:
24. Juli 2018 (online)

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Summary

The following gel filtration properties of the vitamin ढ dependent clotting factors have been established :

1. The elution patterns of factors II, VII, IX, and X in 0.5 M NaCl are the same in factor XII deficient native plasma, anticoagulated plasma, and BaSO4 eluates.

2. In 0.01 M Tris buffer pH 7.3 or 0.5 M NaCl, plasma factors II, IX, and X elute together; factor VII élûtes later as a smaller molecule. All 4 plasma activities elute as larger molecules in 0.01 M Tris buffer and smaller ones in 0.5 M NaCl.

3. In 0.01 M Tris - 0.005 M EDTA peak factor II activity elutes first, followed by factor IX, factor X, and then factor VII.

4. Activation decreases the apparent size of factor IX.

* Present address: Department of Microbiology Dental Faculty, University of Oslo, Oslo, Norway.