Polymorphonuclear Leukocyte-Platelet Interaction: Role of P-Selectin in Thromboxane B2 and Leukotriene C4 Cooperative Synthesis

Norma Maugeri; Virgilio Evangelista; Antonio Celardo; Giuseppe Dell’Elba; Nicola Martelli; Paola Piccardoni; Glovanni de Gaetano; Chiara Cerletti

doi:10.1055/s-0038-1648888

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1994; 72(03): 450-456
DOI: 10.1055/s-0038-1648888

Original Article

Schattauer GmbH Stuttgart

Polymorphonuclear Leukocyte-Platelet Interaction: Role of P-Selectin in Thromboxane B₂ and Leukotriene C₄ Cooperative Synthesis

Norma Maugeri

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

,

Virgilio Evangelista

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

,

Antonio Celardo

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

,

Giuseppe Dell’Elba

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

,

Nicola Martelli

¹Laboratory of Tumor and Vascular Cell Biology, Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, Santa Maria Imbaro, Italy

,

Paola Piccardoni

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

,

Glovanni de Gaetano

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

,

Chiara Cerletti

Giulio Bizzozero Laboratory of Platelet and Leukocyte Pharmacology, Italy

› Institutsangaben

Abstract

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Summary

In PMN/platelet suspensions stimulated by fMLP giant mixed aggregates are formed and TxB₂ and LTC₄ are synthesized as the result of the cooperation in the arachidonic acid (AA) metabolism during cell/cell contact. PMN-derived cathepsin G induced the expression of P-selectin on platelet surface. GE12, an antibody against P-selectin, significantly reduced mixed cell aggregates. GE12 did not affect platelet aggregation induced by PMN-derived supernatants, indicating that the inhibitory effect of GE12 on mixed cell aggregation depends on inhibition of PMN/platelet adhesion. GE12 significantly reduced TxB₂ and LTC₄ production in PMN/platelet mixed cell suspensions stimulated by fMLP. As previously reported, synthesis of ³H-TxB₂ in ³H-AA-labeled PMN/unlabeled platelets indicates that platelets utilize ³H-AA from PMN. ³H-LTC₄ production in unlabeled PMN/³H-AA-labeled platelets indicates that bidirectional routes are utilized in this system for LTC₄ synthesis. GE12 significantly reduced ³H-TxB₂ and ³H-LTC₄ synthesis. These results show that cathepsin G released by activated PMN induces the expression of P-selectin on platelet membrane: this adhesive glycoprotein modulates cell-cell contact and transcellular metabolism of AA.

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Referenzen

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