Thromb Haemost 1978; 40(01): 061-065
DOI: 10.1055/s-0038-1648634
Original Article
Schattauer GmbH Stuttgart

Effects of Autoprothrombin II-A on Epinephrine-Induced Platelet Aggregation of Normal- and Coumadin-Treated Dogs

Gilbert E Herman
The Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, U. S. A.
,
Walter H Seegers
The Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, U. S. A.
,
Raymond L Henry
The Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, U. S. A.
› Author Affiliations
Further Information

Publication History

Received 24 August 1977

Accepted 15 December 1977

Publication Date:
12 July 2018 (online)

Summary

Platelet-rich plasma from dogs and from coumadin-treated dogs aggregated at the same optimum concentration of epinephrine. Neither protein C nor its active form called autoprothrombin II-A was necessary for aggregation of dog platelets with epinephrine. For platelet aggregation, suboptimal concentrations of epinephrine were potentiated by addition of purified autoprothrombin II-A. The latter, by itself, induced platelet aggregation in high concentration.

 
  • References

  • 1 Mammen EF, Thomas WR, Seegers WH. 1960; Activation of purified prothrombin to autoprothrombin I or autoprothrombin II (platelet cofactor II) or autoprothrombin II-A. Thrombosis et Diathesis Haemorrhagica 5: 218
  • 2 Marciniak E, Murano G, Seegers WH. 1967; Inhibitor of blood clooting derived from prothrombin. Thrombosis et Diathesis Haemorrhagica 18: 161
  • 3 Murano G, Seegers WH, Zolton RP. 1974; Autoprothrombin II-A: A competitive inhibitor of autoprothrombin C (factor Xa). A review with additions Thrombosis et Diathesis Haemorrhagica Suppl. 57: 305
  • 4 Mustard JF, Packham MA. 1970; Factors influencin platelet function: adhesion, release and aggregation. Pharmacological Review 22: 97
  • 5 Seegers WH, McCoy LE, Groben HD, Sakuragawa N, Agrawal BB L. 1972; Purification and some properties of autoprothrombin II-A: An anticoagulant perhaps also related to fibrinolysis. Thrombosis Research 7: 443
  • 6 Seegers WH, Hassouna HI, Hewett-Emmett D, Walz DA, Andary TJ. 1975; Prothrombin and thrombin: Selected aspects of thrombin formation, properties, inhibition and immunology. Seminars in Hemostasis 1: 211
  • 7 Seegers WH, Novoa E, Henry RL, Hassouna HI. 1976; Relationship of “new” vitamin K-dependent Protein C and “old” autoprothrombin II-A. Thrombosis Research 8: 543
  • 8 Seiverd CE. 1975. Hematology for Medical Technologists. Lea & Febiger; Philadelphia: p 410
  • 9 Sinakos Z, Caen JP. 1967; Platelet aggregation in mammalians (human, rat, rabbit, guinea pig, horse, dog) a comparative study. Thrombosis et Diathesis Haemorrhagica 17: 99
  • 10 Stenflo J. 1976; A new vitamin K-dependent protein. Journal of Biological Chemistry 251: 355
  • 11 Zolton RP, Seegers WH. 1973; Autoprothrombin II-A: Thrombin removal and mechanism of induction of fibrinolysis. Thrombosis Research 3: 23