Sedimentation and Adhesion of Platelets onto a Horizontal Glass Surface

E Ruckenstein; A Marmur; S. R Rakower

doi:10.1055/s-0038-1648049

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 36(02): 334-342
DOI: 10.1055/s-0038-1648049

Original Article

Schattauer GmbH

Sedimentation and Adhesion of Platelets onto a Horizontal Glass Surface

E Ruckenstein

¹Faculty of Engineering and Applied Sciences and the Department of Surgery, School of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.

,

A Marmur

¹Faculty of Engineering and Applied Sciences and the Department of Surgery, School of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.

,

S. R Rakower

¹Faculty of Engineering and Applied Sciences and the Department of Surgery, School of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.

› Author Affiliations

Abstract

Summary

An experimental method for measuring platelet adhesion to a glass surface and platelet sedimentation rate is described. Anticoagulated platelet-rich plasma is placed on a horizontal glass slide for various contact periods. The number of platelets adhering to the slide per unit area is recorded as a function of time. The experimental results are used to verify theoretical predictions which account for the effect of the slow sedimentation rate of the platelets and for their escape over the potential barrier between them and the solid surface. By a least-squares fit of the theoretical equation to the experimental data, both the platelet adhesiveness, in terms of P the probability to overcome the potential barrier, and the platelet sedimentation rate V are evaluated. A range of values of P and V for healthy humans is presented.

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References

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