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Thromb Haemost 1976; 36(02): 311-318
DOI: 10.1055/s-0038-1648046
Original Article
Schattauer GmbH

Effects of Matrix Contact during Gel Filtration of Human Platelets in Plasma

Jack N. Lindon   *
1   Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass, U.S.A.
,
Robert Rodvien   **
1   Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass, U.S.A.
,
David F. Waugh
1   Department of Biology, Massachusetts Institute of Technology, Cambridge, Mass, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 18 October 1975

Accepted 02 May 1976

Publication Date:
03 July 2018 (online)

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Summary

When gel filtration is used to transfer platelets from plasma into an established environment, alterations in platelet characteristics may result from the change in environment or from the effects of platelet contact with the gel matrix. To approach the problem of evaluating the relative contributions from these sources, a Sepharose 2B matrix was employed and platelets transferred from citrate anticoagulated PRP into autologous PPP to yield plasma-GFP. Platelet recoveries averaged 93 %. PRP: plasma-GFP pairs were found to be indistinguishable with respect to: morphology; ADP, thrombin or collagen-induced aggregation response; uptake of 5-hydroxytryptamine (5-HT) or adenosine; and thrombin or collagen-induced release of accumulated 5-HT or adenosine. Pairs are distinguishable by prostaglandin E2 synthesis assayed immediately after filtration.

* A portion of this work was submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Massachusetts Institute of Technology, 1974.


** Present address: Pacific Medical Center, San Francisco, California.


 

  • References

  • 1 Baughman D.L, Waugh D. F. 1967; Bovine thrombin purification and certain properties. Journal of Biological Chemistry 242: 5252.
    PubMedGoogle Scholar
  • 2 Behrman H. R. 1971; Radioimmunoassay of prostaglandins. The Physiologist 14: 110.
    PubMedGoogle Scholar
  • 3 Born G. V. R. 1962; Aggregation of blood platelets by ADP and its reversal. Nature (London) 194: 927.
    PubMedGoogle Scholar
  • 4 Born G. V. R. 1970; Observations on the change in shape of blood platelets brought about by adenosine diphosphate. Journal of Physiology 209: 487.
    CrossrefPubMedGoogle Scholar
  • 5 Hovig T. 1963; Aggregation of rabbit platelets produced in vitro by saline extract of tendons. Thrombosis et Diathesis Haemorrhagica 9: 248.
    PubMedGoogle Scholar
  • 6 Lages B. A, Scrutton M. C, Holmsen H, Day H. J. 1973; Some characteristics of human platelets isolated by gel filtration. Circulation, Supplement IV to Vols. VII and VIII 55.
    PubMedGoogle Scholar
  • 7 Larrimer N. R, Balcerzak S. P, Wetz E. N, Lee R. E. 1970; Surface structure of normal human platelets. American Journal of Medical Science 259: 242.
    CrossrefPubMedGoogle Scholar
  • 8 Levy-Toledano S, Rendu F, Besson P, Caen J. 1972; Aggregation of human gel filtered platelets. Requirements of apyrase and proteins. Revue Européenne D’Etudes Cliniques et Biologiques 17: 513.
    PubMedGoogle Scholar
  • 9 Marcus A. J. 1969; Platelet function. New England Journal of Medicine 280 (22) 1213 (23)1278 and (24)1330.
    CrossrefPubMedGoogle Scholar
  • 10 Mason R. G, Read M. S, Zucker W. H, Shermer R. W. 1973; Comparison of aggregability and ultrastructure of platelets separated from plasma by various means. (Abstract). Federation Proceedings 32: 844.
    PubMedGoogle Scholar
  • 11 Michal F, Birkin B. G. 1969; Physiological and pharmacological aspects of the platelet. Annual Review of Pharmacology 9: 95.
    CrossrefPubMedGoogle Scholar
  • 12 Morris K, Ashbrook P. C, Didisheim P. 1974; The effect of acetylsalicylic acid (ASA) on gel filtered human platelets. (Abstract). Federation Proceedings 33: 611.
    PubMedGoogle Scholar
  • 13 Mustard J. F, Packham M. A. 1970; Platelet aggregation and the platelet release reaction in thromboembolism. Pharmacological Reviews 22: 97.
    PubMedGoogle Scholar
  • 14 Rosenberg R. D, Waugh D. F. 1970; Multiple bovine thrombin components. Journal of Biological Chemistry 245: 5049.
    PubMedGoogle Scholar
  • 15 Rozenberg M. C, Holmsen H. 1968; Adenine nucleotide metabolism of blood platelets. II. Uptake of adenosine and inhibition of ADP-induced platelet aggregation. Biochimica et Biophysica Acta 155: 342.
    CrossrefPubMedGoogle Scholar
  • 16 Salzman E. W. 1974; Role of platelets in blood-surface interactions. Federation Proceedings 30: 1503.
    PubMedGoogle Scholar
  • 17 Salzman E. W, Lindon J. N, Rodvien R. 1976; Cyclic AMP in human blood platelets: relation to platelet prostaglandin synthesis induced by centrifugation or surface contact. Journal of Cyclic Nucleotide Research 2: 25.
    PubMedGoogle Scholar
  • 18 Tangen O, Berman H. J, Marfey P. 1971; Gel filtration - A new technique for separation of blood platelets. Thrombosis et Diathesis Haemorrhagica 25: 268.
    PubMedGoogle Scholar
  • 19 Tangen O, Berman H. J. 1972; Gel filtration of blood platelets: A methodological report. Advances in Experimental Medicine and Biology 34: 235.
    PubMedGoogle Scholar
  • 20 Tangen O, McKinnon E. L, Berman H. J. 1973a On the fine structure and aggregation characteristics of gel filtered platelets. Scandinavian Journal of Haematology 10: 96.
    PubMedGoogle Scholar
  • 21 Tangen O, Andrae M. L, Nilsson B. E. 1973b Nucleotide leakage from platelets in artificial media: Prevention by albumin and other macromolecules and relation to ADP-induced platelet aggregation. Scandinavian Journal of Haematology 11: 241.
    PubMedGoogle Scholar
  • 22 Wolfe S. M, Shulman N. R. 1970; Inhibition of platelet energy production and release reactions by PGE1, theophylline and cAMP. Biochemistry and Biophysics Research Communications 41: 128.
    CrossrefPubMedGoogle Scholar