Platelet Preservation. III. The Influence of Dimethylsulfoxide and Cooling Conditions on Serotonin Uptake Velocity and Response to Hypotonic Stress of Human Platelets
06 November 1975
Accepted 26 February 1976
03 July 2018 (online)
1. Serotonin uptake velocity of human platelets was found to be reversibly inhibited by the presence of dimethylsulfoxide (DMSO).
2. Incubation of platelet concentrates in the presence of DMSO for 90 minutes at room temperature decreased serotonin uptake velocity and response to hypotonic stress.
3. The serotonin uptake velocity was not substantially influenced by the temperature of incubation with DMSO.
4. The best recovery of serotonin uptake velocity after cryopreservation in the presence of DMSO was observed when DMSO was added quickly before cooling and removed quickly after thawing.
5. No significant differences were observed in serotonin uptake velocity and response to hypotonic stress after cryopreservation in the presence of DMSO when cooling rates were varied from 2 to 10° C/min.
- 1 Born G. V. R, Gillson R. E. 1959; Studies on the uptake of 5-hydroxytryptamine by blood platelets. Journal of Physiology 146: 472.
- 2 Handin R. I, Fortier N. L, Valeri C. R. 1970; Platelet response to hypotonic stress after storage at 4 C and 22 C. Transfusion 10: 305.
- 3 Handin R. I, Valeri C. R. 1972; Improved viability of previously frozen platelets. Blood 40: 509.
- 4 Hardeman M. R. 1975; The effect of continuous dialysis on platelet preservation. Transfusion 15: 231.
- 5 Hardeman M. R, Heynens C. J. L. 1974a The active serotonin uptake and the hypotonic shock response of human blood platelets. Methods, normal values and the influence of various experimental conditions. Thrombosis et Diathesis Haemorrhagica 32: 391.
- 6 Hardeman M. R, Heynens C. J. L. 1974b Storage of human blood platelets. The serotonin uptake and hypotonic shock response as in vitro viability tests. Thrombosis et Diathesis Haemorrhagica 32: 405.
- 7 Hardeman M. R, Prins H. K. 1975 Serotonin uptake and glycolytic activity of human platelets after prolonged incubation with glucose-poor plasma. Haemostasis. in press.
- 8 Kim B. K, Baldini M. G. 1973; Preservation of viable platelets by freezing. Effect of plastic containers. Proceedings of the Society for Experimental Biology and Medicine 142: 345.
- 9 Kim B. K, Baldini M. G. 1974a Biochemistry, function, and hemostatic effectiveness of frozen human platelets. Proceedings of the Society for Experimental Biology and Medicine 145: 830.
- 10 Kim B. K, Baldini M. G. 1974b The platelet response to hypotonic shock. Its value as an indicator of platelet viability after storage. Transfusion 14: 130.
- 11 Lundberg A, Murphy S. 1972; Survival in vivo of human blood platelets frozen without protective additives. Scandinavian Journal of Haematology 9: 222.
- 12 Murphy S, Sayar S. N, Abdou N. L, Gardner F. H. 1974; Platelet preservation by freezing. Use of dimethylsulfoxide as cryoprotective agent. Transfusion 14: 139.
- 13 Odink J. 1975; Platelet preservation. II. The response of human platelet suspensions to hypotonic stress. Thrombosis and Haemostasis 36: XXX.
- 14 Odink J, Sprokholt R. 1975 Platelet preservation. I. The use of decrease in light absorbance as a screening method in cryopreservation studies on human platelets. Submitted for publication.
- 15 Valeri C. R. 1974; Hemostatic effectiveness of liquid-preserved and previously frozen human platelets. New England Journal of Medicine 290: 353.
- 16 Valeri C. R, Feingold H, Marchionni L. D. 1974a A simple method for freezing human platelets using 6% dimethylsulfoxide and storage at —80° C. Blood 43: 131.
- 17 Valeri C. R, Feingold H, Marchionni L. D. 1974b The relation between response to hypotonic stress and the 51Cr recovery in vivo of preserved platelets. Transfusion 14: 331.