Thromb Haemost 1976; 36(01): 104-114
DOI: 10.1055/s-0038-1648014
Original Article
Schattauer GmbH

The Activation of Human Factor X in Sodium Citrate: the Role of Factor VII[*]

D. L Aronson
1  Bureau of Biologics, Food and Drug Administration, Bethesda, MD 20014, USA
,
A. J Mustafa
1  Bureau of Biologics, Food and Drug Administration, Bethesda, MD 20014, USA
› Author Affiliations
Further Information

Publication History

Received 26 September 1975

Accepted 04 January 1976

Publication Date:
03 July 2018 (online)

Summary

Human factor X was purified by several different procedures yielding products which had varying amounts of factor VII and factor IX. Treatment with CHC13 during the fractionation of the factor X removed 95% of the factor VII and factor IX activity and the resulting factor X activated more slowly when incubated in 25% sodium citrate. Removal of residual factor VII by DEAE cellulose chromatography yielded a factor X which activated still more slowly and less completely. When the factor VII, removed by chromatography, was added to the chromatographed factor X, the ability to be activated in 25% sodium citrate was restored. Confirmatory evidence for the role of factor VII in this reaction was the inhibition of the conversion of the factor X by both DFP and SBTI.

* This work was presented at the American Hematology Society’s 1971 meeting.