Thromb Haemost 1976; 36(01): 014-026
DOI: 10.1055/s-0038-1648005
Original Article
Schattauer GmbH

Molecular Size Distribution of Fibrinogen Derivatives Formed in Vitro and in Vivo: a Chromatographic Study

M. B Donati*
1  Laboratory of Blood Coagulation, Medical Research Department, University of Leuven, Belgium
,
R Verhaeghe
1  Laboratory of Blood Coagulation, Medical Research Department, University of Leuven, Belgium
,
D. E Culasso**
1  Laboratory of Blood Coagulation, Medical Research Department, University of Leuven, Belgium
,
J Vermylen
1  Laboratory of Blood Coagulation, Medical Research Department, University of Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 25 September 1975

Accepted 02 April 1976

Publication Date:
03 July 2018 (online)

Summary

Using gel chromatography, fibrinogen derivatives present in purified systems or in biological fluids were separated and partially characterized. Eight groups of fibrinogen derivatives could be separated by gel filtration through 6% agarose in large columns, four with an elution volume smaller and four groups with an elution volume larger than that of fibrinogen. Careful calibration of the column allowed estimation of the diffusion coefficients of some of the derivatives and, thus, comparison with derivatives previously identified. Three, rather than two, groups of intermediate derivatives were observed during the degradation of human fibrinogen by plasmin in vitro or in vivo. One of these had a marked tendency to polymerize.

A rather distinct difference in elution pattern was found between plasma obtained during streptokinase administration and from patients with intravascular coagulation.

* Present address: Laboratory for Haemostasis and Thrombosis Research, ‘Mario Negri’ Institute, Via Eritrea, 62 – 20157 Milano, Italy.


** Present address: Avellaneda 2127, Cordoba, Argentina.