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Thromb Haemost 1974; 32(01): 232-238
DOI: 10.1055/s-0038-1647688
Original Article
Schattauer GmbH

Electronic Platelet Counting after Isopycnic Differential Centrifugation A Simple Rapid and Accurate Method

M De Bruyere
1   Department of Haematology of the University of Louvain ( Belgium)
,
CL Bellenot
1   Department of Haematology of the University of Louvain ( Belgium)
,
G Sokal
1   Department of Haematology of the University of Louvain ( Belgium)
› Author Affiliations
Further Information

Publication History

Received 15 February 1974

Accepted after resubmission 09 May 1974

Publication Date:
30 June 2018 (online)

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Summary

A semi-automatized platelet counting procedure with the use of a Coulter Counter is described. Its originality consists in providing an electronic method of counting of the platelets after a differential centrifugation of the blood sample previously diluted with a hypertonic NaCl solution (density 1.053) which is isopycnic to platelet density. This procedure allows elimination of red and white cells without loss of platelets in the supernatant. The final platelet number can be calculated without further corrections.

The method is quick and does not require specially skilled technicians. A single technician can perform 40 counts in one hour. The reproductibility of the method is excellent and the coefficient of variation 2,4 %.

The accuracy and feasibility of the method is also sustained by two years’ experience in an haematological routine laboratory.

 

  • References

  • 1 Boedewich P. H. 1965; Electronic Counting of Blood Platelets. Postgraduate Medicine 30: 44.
    PubMedSearch in Google Scholar
  • 2 Brecher G, Schneiderman M, Cronkite E. P. 1953; The Reproductibility and Constancy of the Platelet Count. American Journal of Clinical Pathology 23: 15.
    CrossrefPubMedSearch in Google Scholar
  • 3 Bull B. S, Schneiderman M. A, Brecher G. 1965; Platelet Counts with the Coulter Counter. American Journal of Clinical Pathology 44: 678.
    CrossrefPubMedSearch in Google Scholar
  • 4 Cartier P, Marchand J. C, Garrean H. 1969; La separation des cellules sanguines. Annales de Biologie Clinique 27: 133.
    PubMedSearch in Google Scholar
  • 5 D’Angelo A RT, Lacombe M. 1963; Décompte des Plaquettes à l’aide d’un compteur électronique. Canadian Journal of Medical Technology 25: 182.
    PubMedSearch in Google Scholar
  • 6 Davis R. E, Green R. E. 1967; Automatic platelet counting with the Coulter particle counter. Journal of Clinical Pathology 20: 111.
    PubMedSearch in Google Scholar
  • 7 Eastham R. D. 1965; Simple method for Separating Platelets from Red Cells before enumeration with an electronic Counter. Journal of Clinical Pathology 18: 248.
    CrossrefPubMedSearch in Google Scholar
  • 8 Eastham R. D, Morgan F. H. 1967; Comparison of Platelet Count Methods. Journal of Medical and Laboratory Technology 24: 315.
    PubMedSearch in Google Scholar
  • 9 Eggleton M. G, Sharp A. A. 1963; Platelet Counting using the Coulter Electronic Counter. Journal of Clinical Pathology 16: 164.
    CrossrefPubMedSearch in Google Scholar
  • 10 Girling J. H. 1962; An Automatic Platelet Counting Technique. Journal of Medical and Laboratory Technology 19: 168.
    PubMedSearch in Google Scholar
  • 11 Mayer K. E, Mink B. 1968; Slide Rule Calculator for Platelet Enumeration with the Coulter Electronic Particle Counter. American Journal of Clinical Pathology 49: 453.
    CrossrefPubMedSearch in Google Scholar
  • 12 Mayne E. E, Carville J. M. 1969; Enumeration of Platelets using a model B Counter. Journal of Clinical Pathology 122: 107.
    PubMedSearch in Google Scholar
  • 13 Simmons H, Schwabbauer M. L, Earhart C. A. 1971; Automated platelet Counting with the Autoanalyser. Journal of Laboratory of Clinical Medicine 77: 656.
    PubMedSearch in Google Scholar
  • 14 Williamson J. R. 1966. A practical integrated method for the electronic counting of platelets, leucocytes and erythrocytes. In: Baker F. S. (ed.) Advances in Medical Laboratory Technology, IV. Butterworth.:
    Search in Google Scholar