Effect of a Moderate Fish lntake on Platelet Aggregation in Human Platelet-Rich Plasma

A C v Houwelingen; A A H M Hennissen; F Verbeek-Schippersa; T Simonsen; A D M Kester; G Hornstra

doi:10.1055/s-0038-1647525

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1988; 59(03): 507-513
DOI: 10.1055/s-0038-1647525

Original Article

Schattauer GmbH Stuttgart

Effect of a Moderate Fish lntake on Platelet Aggregation in Human Platelet-Rich Plasma

A C v Houwelingen

¹The Department of Human Biology, Limburg University, Maastricht, The Netherlands

,

A A H M Hennissen

¹The Department of Human Biology, Limburg University, Maastricht, The Netherlands

,

F Verbeek-Schippersa

⁴The TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands

,

T Simonsen

⁵The Department of Medicine, University Hospital, University of Tromsø, Norway

,

A D M Kester

²The Department of Medical Informatics and Statistics, Limburg University, Maastricht, The Netherlands

,

G Hornstra

³The Department of Biochemistry, Limburg University, Maastricht, The Netherlands

› Author Affiliations

Abstract

PDF Download

Summary

This paper describes the results of an international study to investigate the effect of a reasonable amount of dietary fish on platelet aggregation in platelet-rich plasma (PRP) induced by collagen and thrombin. In Maastricht, Tiomsø, and Zeist two groups of healthy male volunteers were given a daily dietary supplement consisting of 135 g of canned mackerel paste (experimental group, n = 40) or meat paste (control group, n = 42) for a 6-week period. Compliance, calculated on the basis of the urinary excretion of lithium, added to the supplements, was about 80%. Platelet number in PRP decreased significantly in the fish group. Collagen-induced platelet aggregation in PRP differed widely between the three centres despite the attempt to use exactly the same conditions. Nonetheless, aggregation decreased significantly in the fish group. The mackerel effect on thrombin-induced aggregation was inconsistent.

Keywords

Fish - Diet - Compliance - Platelte aggregation - Platelte number - PRP - Collagen - Thrombin

PDF (1300 kb)

References

References
1 Hornstra G. Effect of Fish Oil Feeding on Arterial Thrombosis, Platelet Function and Blood Coagulation. In: Dietary fats, prostanoids and arterial thrombosis. Martinus Nijhoff Pulishers; The Hague: 1982. pp 106-37
2 Hornstra G. The significance of fish and fish-oil enriched food for prevention and therapy of ischaemic cardiovascular disease. In: The Role of Fat in Human Nutrition II. Vergroesen AJ, Crawford MA. (eds) Academic Press; London: (in press)
3 Ardlie NG, Han P. Enzymatic basis for platelet aggregation and release: the significance of the “platelet atmosphere” and the relationship between platelet function and blood coagulation. Br J Haematol 1974; 26: 331-356
4 Hornstra G. Platelet-vessel wall interaction: role of blood clotting. Philos Trans R Soc London Ser B 1981; 294: 355-371
5 Mustard JF, Packham MA. Factors influencing platelet function: adhesion, release and aggregation. Pharmacol Rev 1970; 22: 97-187
6 Weiss HJ. Platelets Pathophysiology and Antiplatelet Drug Therapy. Alan R Liss Inc; New York: 1982. Chapter 1 1-26
7 Brox JH, Killie JE, Gunnes S, NordØy A. The effect of cod-liver oil and corn oil on platelets and vessel wall in man. Thromb Haemostas 1981; 46: 604-611
8 Brox JH, Killie JE, Østerud B, Holme S, NordØ A. Effects of cod-liver oil on platelets and coagulation in familial hypercholesterolemia (Type IIa). Acta Med Scand 1983; 213: 137-144
9 Driss F, Vericel E, Lagarde M, Dechavanne M, Darcet P. Inhibition of platelet aggregation and thromboxane synthesis after intake of small amount of icosapentaenoic acid. Thromb Res 1984; 36: 389-396
10 Goodnight Jr SH, Harris WS, Connor WE. The effect of dietary n-3 fatty acids on platelet composition and function in man. a prospective, controlled study Blood 1981; 58: 880-885
11 Mortensen JZ, Schmidt EB, Nielsen AH, Dyerberg J. The effect of (n-6) and (n-3) polyunsaturated fatty acids on hemostasis, blood lipids and blood pressure. Thromb Haemostas 1983; 50: 543-546
12 Sanders TA B, Hochland MC. A comparison of the influence on plasma lipids and platelet function of supplements of n-3 and n-6 polyunsaturated fatty acids. Br J Nutr 1983; 50: 521-529
13 Adam O, Wolfram G, Zoellner N. Vergleich der Wirkung von Linolensäure und Eicosapentaensäure auf die Prostaglandinbiosyn-these und Thrombozytenfunktion beim Menschen. (Comparison of the effect of linolenic acid and eicosapentaenoic acid on prostaglandin biosynthesis and thrombocyte function in humans) Klin Wochenschr 1986; 64 (06) 272-280
14 von SchackyC, Weber PC. Metabolism and effects on platelet function of the purified eicosapentaenoic and docosahexaenoic acids in humans. J Clin Invest 1985; 76 (06) 2446-2450
15 Bolton-Smith C, Gibney MJ. The effect of a dietary supplement of marine oil in healthy volunteers on platelet lipids, platelet function and platelet plasma membrane fluidity. Proc Nutr Soc 1986; 45: 12
16 Dyerberg J. Platelet-vessel wall interaction: influence of diet. Philos Trans R Soc London Ser B 1980; 294: 373-381
17 Hirai A, Terano T, Hamazaki T, Sajiki J, Kondo S, Ozawa A, Fujita T, Miyamoto T, Tamura Y, Kamugai A. The effect of the oral administration of fish oil concentrate on the release and the metabolism of [¹⁴C] arachidonic acid and [¹⁴C] eicosapentaenoic acid by human platelets. Thromb Res 1982; 28: 285-298
18 Hirai A, Terano T, Tamura Y, Yoshida S, Kamugai A. Eicosapentaenoic acid and thrombotic disorders – Epidemiological and clinical approach. Proceedings of the (n-3) Fatty Acid Meeting, Reading, England. 07/1984
19 Lorentz R, Spengler U, Fischer S, Duhm J, Weber P. Platelet function, thromboxane formation and blood pressure control during supplementation of the western diet with cod-liver oil. Circulation 1983; 67: 504-511
20 Nagakawa Y, Orimo H, Harazawa M, Morita I, Yashiro K, Murot S. Effect of eicosapentaenoic acid on the platelet aggregation and composition of fatty acid in man. Atherosclerosis 1983; 47: 71-75
21 NordØy A, Lagarde M, Renaud S. Platelets during alimentary hyperlipaemia induced by cream and cod-liver oil. Eur J Clin Invest 1984; 14: 339-345
22 Sanders TA B, Lovat L, Mistry M, Upton K. Influence of a mackerel diet on plasma lipoproteins and platelet function. Proc Nutr Soc 1986; 45: 5
23 Sanders T, Roshanai F. The influence of different types of n-3 polyunsaturated fatty acids on blood lipids and platelet function in healthy volunteers. Clin Sci 1983; 64: 91-99
24 von Schacky C, Fischer S, Weber PC. Long-term effects of dietary marine n-3 fatty acids upon plasma and cellular lipids, platelet function, and eicosanoid formation in humans. J Clin Invest 1985; 76: 1626-1631
25 Siess W, Scherer B, Böhlig B, Roth P, Kurzmann I, Weber PC. Platelet-membrane fatty acids, platelet aggregation, and thromboxane formation during a mackerel diet. Lancet 1980; 1: 441-444
26 Terano T, Hirai A, Hamazaki T, Kobayashi S, Fujita T, Tamura Y, Kumagai A. Effect of oral administration of highly purified eicosapen-taenoic acid on platelet function, blood viscosity and red cell deformability in healthy human subjects. Atherosclerosis 1983; 46: 321-331
27 Thomgren M, Gustafson A. Effects of 11-week increase in dietary eicosapentaenoic acid on bleeding time, lipids and platelet aggregation. Lancet 1981; 2: 1190-1193
28 Thomgren M, Shafi S, Born GV R. Delay in primary haemostasis produced by a fish diet without change in local thromboxane A2. Br J Haematol 1984; 58: 567-578
29 Rylance PB, Gordge MP, Saynor R, Parsons V, Weston MJ. Fish oil modifies lipids and reduces platelet aggregability in haemodialysis patients. Nephron 1986; 43 (03) 196-202
30 Bradlow BA, Chetty N, van de WesthuyzenJ, Mandelsohn D, Gibson JE. The effects of a mixed fish diet on platelet function, fatty acid and serum lipids. Thromb Res 1983; 29 (06) 561-568
31 Atkinson PM, Wheeler MC, Mendelsohn D, Pienaar N, Chetty N. Effects of a 4-week freshwater fish (trout) diet on platelet aggregation, platelet fatty acids, serum lipids, and coagulation factors. Am J Hematol 1987; 24 (02) 143-149
32 Tilvis RS, Rasi V, Viinikka L, Ylikorkala O, Miettinen TA. Effects of purified fish oil on platelet lipids and function in diabetic women. Clin Chim Acta 1987; 164 (03) 315-322
33 van HouwelingenAC, Nordøy A, van der BeekE, Houtsmuller UM T, de MetzM, Hornstra G. Effect of a moderate fish intake on blood pressure, bleeding time, hematology and clinical chemistry. Am J Clin Nutr 1987; 46: 436-437
34 Sanchez-Castill CP, James WP T. The use of lithium as a marker of sodium intake. Proc Nutr Soc 1983; 42: 168
35 Dixon WJ. Ratios involving extreme values. Ann Math Stat 1951; 22: 68-78
36 Miller RG. Simultaneous Statistical Inference. 2nd. ed Springer Verlag; New York: 1981
37 van HouwelingenA C, Hornstra G. Effect of a moderate fish intake on blood pressure, platelet function and safety aspects. Agents Actions 1987; 22: 3-4
38 Burck JW, Magerius PW. The role of prostaglandins in platelet function. Sem Haematol 1979; 16: 196-207
39 Ahmed AA, Holub BJ. Alternation and recovery of bleeding times, platelet aggregation and fatty acid composition of individual phospholipids in platelets of human subjects receiving a supplement of cod-liver oil. Lipids 1984; 19: 617-624
40 Weiss HJ. Platelets Pathophysiology and Antiplatelet Drug Therapy. Alan R Liss Inc; New York: 1982. Chapter 4 76-94
41 Kinlough-Rathbone RL, Packham MA, Mustard JF. Platelet aggregation. In: Measurements of Platelet Function. Harker LA, Zimmerman TS. (eds) Churchill Livingstone; Edinburgh: 1983. pp 64-91