Thromb Haemost 1991; 65(01): 073-076
DOI: 10.1055/s-0038-1647457
Original Article
Schattauer GmbH Stuttgart

Antithrombotic Mechanisms of Urokinase lmmobilized Polyurethane

Y Kitamoto
1   The Third Department of Internal Medicine, Kumamoto University Medical School, Kumamoto, Japan
,
M Tomita
1   The Third Department of Internal Medicine, Kumamoto University Medical School, Kumamoto, Japan
,
S Kiyama
1   The Third Department of Internal Medicine, Kumamoto University Medical School, Kumamoto, Japan
,
T Inoue
1   The Third Department of Internal Medicine, Kumamoto University Medical School, Kumamoto, Japan
,
Y Yabushita
2   The Research and Development Center, Unitika Ltd, Kyoto, Japan
,
T Sato
3   The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan
,
H Ryoda
3   The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan
,
T Sato
1   The Third Department of Internal Medicine, Kumamoto University Medical School, Kumamoto, Japan
› Author Affiliations
Further Information

Publication History

Received 08 August 1989

Accepted after revision 03 August 1990

Publication Date:
02 July 2018 (online)

Summary

Urokinase immobilized polymer is highly antithrombotic, which cannot be explained only by fibrinolysis. We immobllized 10 IU/cm2 of urokinase to polyurethane by using maleic anhydride methylvinyl ether copolymer as a carrier. Then we incubated blood in circular tubes made of this material, measured the clotting factors and observed the surface of the tubes after incubation by scanning electronmicroscopy and immunofluorescence microscopy. After 5 min incubation, the relative activities of factors V, VIII, IX, X and XII, fibrinogeil, plasminogen and α2 plasmin inhibitor decreased, but the activity of factor VII increased. No platelet adhesion to the surface of the urokinase immobilized polyurethane was observed and there was no significant adsorption of serum proteins, including fibrinogen, fibronectin and vWF antigen, or the surface. Urokinase-immobilized polyurethane catalyzed the digestion of clotting factors as well as fibrinolysis and also inhibited platelet adhesion on its surface probably by inhibiting protein adsorption and its clinical application including vessel prosthesis should be developed further.

 
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