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Thromb Haemost 1990; 64(01): 121-123
DOI: 10.1055/s-0038-1647266
Original Article
Schattauer GmbH Stuttgart

Solvent Extraction of Test Plasmas for Improved Recovery of Lupus Anticoagulant Activity

Thomas Exner
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
,
George Papadopoulos
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
,
Nilgun Sahman
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
,
Jerry Koutts
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
› Author Affiliations
Further Information

Publication History

Received 18 July 1989

Accepted after revision 15 March 1990

Publication Date:
25 July 2018 (online)

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Summary

Phospholipid procoagulant material mainly derived from platelets interferes or “bypasses” the more specific tests for lupus anticoagulants. Such material in test plasmas can be inactivated with recovery of lupus anticoagulant activity by simple extraction with chloroform. This solvent treatment damages mainly factors VIII, V, VII and IX. Ether and various other solvents were less damaging to these clotting factors but not quite as effective as chloroform in the recovery of lupus anticoagulant activity.

 

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