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Thromb Haemost 1990; 64(01): 121-123
DOI: 10.1055/s-0038-1647266
Original Article
Schattauer GmbH Stuttgart

Solvent Extraction of Test Plasmas for Improved Recovery of Lupus Anticoagulant Activity

Thomas Exner
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
,
George Papadopoulos
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
,
Nilgun Sahman
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
,
Jerry Koutts
The Hematology Department, Westmead Hospital, Westmead, Sydney, Australia
› Institutsangaben
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Publikationsverlauf

Received 18. Juli 1989

Accepted after revision 15. März 1990

Publikationsdatum:
25. Juli 2018 (online)

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Summary

Phospholipid procoagulant material mainly derived from platelets interferes or “bypasses” the more specific tests for lupus anticoagulants. Such material in test plasmas can be inactivated with recovery of lupus anticoagulant activity by simple extraction with chloroform. This solvent treatment damages mainly factors VIII, V, VII and IX. Ether and various other solvents were less damaging to these clotting factors but not quite as effective as chloroform in the recovery of lupus anticoagulant activity.

 

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