Autoradiographic Observation of Platelets in Cerebrovascular Injuries Induced by Arachidonic Acid and its Prevention by Ticlopidine

Tsukasa Fujimoto; Hidenori Suzuki; Kenjiro Tanoue; Yoshiharu Fukushima; Hiroh Yamazaki

doi:10.1055/s-0038-1647053

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1988; 60(02): 319-323
DOI: 10.1055/s-0038-1647053

Original Article

Schattauer GmbH Stuttgart

Autoradiographic Observation of Platelets in Cerebrovascular Injuries Induced by Arachidonic Acid and its Prevention by Ticlopidine

Authors

Tsukasa Fujimoto

The Department of Neurosurgery, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, and Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Hidenori Suzuki

The Department of Neurosurgery, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, and Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Kenjiro Tanoue

The Department of Neurosurgery, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, and Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Yoshiharu Fukushima

The Department of Neurosurgery, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, and Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Hiroh Yamazaki

The Department of Neurosurgery, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, and Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

Abstract

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Summary

The behavior of circulating ¹¹¹In-labeled platelets in cerebrovascular injuries induced by arachidonic acid injection was studied. Fourteen rabbits were pretreated with the antiplatelet agent ticlopidine, and 10 rabbits were used for controls. Arachidonic acid (AA, 0.7 mg/kg) was injected into the internal carotid artery. Prior to the injection, platelets labeled with ¹¹¹In- oxine were injected for autoradiography of the brain. Evans blue was injected as an indicator of blood-brain barrier disturbance. Nine control animals showed marked blue staining, and one showed slight blue staining. Seven out of the 14 pretreated animals showed slight or no staining, while 7 showed intensive staining. The distributions of the blue staining and the radioactivity showed high correlation. In the rabbits whose platelet aggrega- bility was depressed by ticlopidine, lower blue staining as well as lower radioactivity was observed. Our findings suggest that activated platelets have an important role in the genesis of cerebrovascular injuries.

Keywords

¹¹¹In-oxine labeled platelet - Autoradiogram - Arachidonic acid - Cerebrovascular injury - Ticlopidine

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References

References
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