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Thromb Haemost 1988; 60(03): 514-517
DOI: 10.1055/s-0038-1647002
Original Article
Schattauer GmbH Stuttgart

Interaction of Thrombin-Stimulated Platelets with Vitronectin (S-Protein of Complement) Substrate: Inhibition by a Monoclonal Antibody to Glycoprotein IIb-IIIa Complex

Perumal Thiagarajan
The Department of Medicine, Division of Hematology, University of Washington, Seattle, WA, USA
,
Kathleen Kelly
The Department of Medicine, Division of Hematology, University of Washington, Seattle, WA, USA
› Author Affiliations
Further Information

Publication History

Received 25 April 1988

Accepted after revision 23 August 1988

Publication Date:
30 June 2018 (online)

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Summary

Platelets adhere to vitronectin substrate following activation with physiological concentrations of thrombin. Adhesion of activated platelets to vitronectin substrate is dependent upon the presence of divalent cations, the amount of vitronectin, and the duration of adhesion assay. The adhesion of platelets is inhibited by synthetic peptides containing the sequence of Arg-Gly-Asp. In addition, monoclonal antibodies to glycoprotein IIb-IIIa complex inhibit the adhesion of activated platelets to vitronectin substrate in a dose-dependent manner. These studies suggest that the glycoprotein IIb-IIIa complex on activated platelets may interact with vitronectin substrate through the Arg-Gly-Asp mechanism. Since vitronectin is present in the subendothelial matrix, it might be involved in platelet-vessel wall interactions

Keywords

Platelet adhesion - Vitronectin - Glycoprotein IIb and IIIa
 

  • References

  • 1 Baenziger NL, Majerus PW. Isolation of human platelet and platelet membranes. Methods Enzymol 1974; 31: 149-155
    PubMedGoogle Scholar
  • 2 Barnes DW, Silnutzer J. Isolation of human serum spreading factor. J Biol Chem 1983; 258: 12548-12552
    PubMedGoogle Scholar
  • 3 Bastida E, Escolar G, Ordinas A, Sixma JJ. Fibronectin is required for platelet adhesion and for thrombus formation on subendothelium and collagen surfaces. Blood 1987; 70: 1437-1442
    PubMedGoogle Scholar
  • 4 Bennett JS, Vilaire G, Cines DB. Identification of the fibrinogen receptor on human platelets by photoaffinity labelling. J Biol Chem 1982; 257: 8049-8054
    PubMedGoogle Scholar
  • 5 Dahlbäck B, Podack ER. Characterization of human S protein, an inhibitor of the membrane attack complex of complement. Demonstration of a free reactive thiol group. Biochemistry 1985 24. 2368-2374
    PubMedGoogle Scholar
  • 6 Dahlbäck K, Lofberg H, Dahlbäck B. Localization of vitronectin (S-protein of complement) in normal human skin. Acta Derm Venereol 1986; 66: 461-467
    PubMedGoogle Scholar
  • 7 Engvall E, Ruoslahti E. Binding of soluble form of fibroblast surface protein, fibronectin to collagen. Int J Cancer 1977; 20: 1-5
    CrossrefPubMedGoogle Scholar
  • 8 Gardner JM, Hynes RO. Interaction of fibronectin with its receptor on platelets. Cell 1985; 42: 439-448
    CrossrefPubMedGoogle Scholar
  • 9 Gebb C, Hayman EG, Engvall E, Ruoslahti E. Interaction of vitronectin with collagen. J Biol Chem 1986; 261: 16698-16703
    PubMedGoogle Scholar
  • 10 Gogstad GO, Brosstad F, Krutness MB, Hagen I, Solum NO. Fibrinogen-binding properties of the human platelet glycoprotein IIb-IIIa complex: A study using crossed radioimmunoelectrophoresis. Blood 1982; 60: 663-671
    PubMedGoogle Scholar
  • 11 Haverstick DM, Cowan JF, Yamada KM, Santoro SA. Inhibition of platelet adhesion to fibronectin, fibrinogen and von Willebrand factor substrates by a synthetic tetrapeptide derived from the cell binding domain of fibronectin. Blood 1985; 66: 946-952
    PubMedGoogle Scholar
  • 12 Hayman EG, Pierschbacher MD, Ohgren Y, Ruoslahti E. Serum spreading factor (vitronectin) is present at the cell surface and in tissues. Proc Natl Acad Sci USA 1983; 80: 4003-4007
    CrossrefPubMedGoogle Scholar
  • 13 Holmes R. Preparation from human serum of an alpha-one protein which induces the immediate growth of unadapted cells in vitro. J Cell Biol 1967; 32: 297-308
    CrossrefPubMedGoogle Scholar
  • 14 Houdijk WP M, Sixma JJ. Fibronectin in artery subendothelium is important for platelet adhesion. Blood 1985; 65: 598-604
    PubMedGoogle Scholar
  • 15 Jenne D, Stanley KK. Molecular cloning of S-protein, a link between complement, coagulation and substrate adhesion. EMBO J 1986; 4: 3153-3157
    PubMedGoogle Scholar
  • 16 Nachman RL, Leung LL K. Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen. J Clin Invest 1982; 69: 263-269
    CrossrefPubMedGoogle Scholar
  • 17 Nievelstein PF E M, Sixma JJ. Glycoprotein IIb-IIIa and RGD(S) are not important for fibronectin-dependent platelet adhesion under flow conditions. Blood 1988; 72: 82-88
    PubMedGoogle Scholar
  • 18 Plow EF, McEver RP, Coller BS, Woods VL, Marguerie GA, Ginsberg MS. Related binding mechanisms for fibrinogen, fibronectin, von Willebrand factor and thrombospondin or thrombin-stimulated human platelets. Blood 1985; 66: 724-727
    PubMedGoogle Scholar
  • 19 Podack ER, Dahlbäck B, Griffin JH. Interaction of S protein of complement with thrombin and antithrombin III during coagulation. J Biol Chem 1986; 261: 7387-7392
    PubMedGoogle Scholar
  • 20 Preissner KT, MÜller-Bergaus K. Neutralization and binding of heparin by S protein/vitronectin in the inhibition of factor Xa by antithrombin III. J Biol Chem 1987; 262: 12247-12253
    PubMedGoogle Scholar
  • 21 Preissner KT, Heimburger N, Anders E, Miiller-Berghaus G. Immunochemical and functional comparison of human S-protein and vitronectin. Evidence for the identity of both plasma protein. Biochem Biophys Res Commun 1986 134. 951-956
    PubMedGoogle Scholar
  • 22 Preissner KT, Wassmuth R, MÜller-Bergaus G. Physicochemical characterization of human S protein and its functions in blood coagulation system. Biochem J 1986; 231: 349-345
    PubMedGoogle Scholar
  • 23 Pytela R, Pierschbacher MD, Ginsberg MH, Plow EF, Ruoslahti E. Platelet membrane glycoprotein IIb/IIIa: Member of a family of Arg-Gly-Asp-specific adhesion receptors. Science 1986; 231: 1559-1562
    CrossrefPubMedGoogle Scholar
  • 24 Ruggeri ZM, Bader R, DeMarco L. Glanzmann thrombasthenia: Deficient binding of von Willebrand factor to thrombin-stimulated platelets. Proc Natl Acad Sci USA 1982; 79: 6038-6041
    CrossrefPubMedGoogle Scholar
  • 25 Ruggeri ZM, DeMarco L, Gatti L, Bader R, Montgomery RR. Platelets have more than one binding site for von Willebrand factor. J Clin Invest 1983; 72: 01-12
    CrossrefPubMedGoogle Scholar
  • 26 Ruoslahti E, Hayman EG, Pierschbacher MD. Extracellular matrices and cell adhesion. Arteriosclerosis 1985; 5: 581-594
    CrossrefPubMedGoogle Scholar
  • 27 Sakariassen KS, Bolhuis PA, Sixma JJ. Human blood platelet adhesion to artery subendothelium is mediated by factor VIII-von Willebrand factor bound to the subendothelium. Nature 1979; 279: 636-638
    CrossrefPubMedGoogle Scholar
  • 28 Sakariassen KS, Nievelstein PF E M, Coller BS, Sixma JJ. The role of platelet membrane glycoproteins Ib and IIb-IIIa in platelet adherence to human artery subendothelium. Br J Haematol 1986; 63: 681-691
    CrossrefPubMedGoogle Scholar
  • 29 Suzuki S, Piersbacher MD, Hayman EG, Nguyen K, Ohgren Y, Ruioslahti E. Domain structure of vitronectin. Alignment of active sites. J Biol Chem 1984 259. 15307-15314
    PubMedGoogle Scholar
  • 30 Suzuki S, Oldberg A, Hayman EG, Pierschbacher MD, Ruoslahti E. Complete amino acid sequence of human vitronectin deduced from cDNA. Similarity of cell attachment sites in vitronectin and fibronectin. EMBO J 1985 4. 2519-2524
    PubMedGoogle Scholar
  • 31 Thiagarajan P, Kelly K. Exposure of binding sites for vitronectin on platelets following stimulation. J Biol Chem 1988; 3035-3038
    PubMedGoogle Scholar
  • 32 Tschopp T, Weiss HJ, Baumgartner HR. Decreased adhesion of platelets to subendothelium in von Willebrand’s disease. J Lab Clin Med 1974; 83: 296-300
    PubMedGoogle Scholar
  • 33 Turitto VT, Weiss HJ, Zimmerman T, Sussman I. Factor VIII/von Willebrand factor in subendothelium mediates platelet adhesion. Blood 1985; 65: 823-831
    PubMedGoogle Scholar
  • 34 Weiss HJ, Tschopp TB, Baumgartner HR, Sussman II, Johnson MM, Egan JJ. Decreased adhesion of giant (Bernard-soulier) platelets to subendothelium-further implication on the role of the von Willebrand factor in hemostasis. Am J Med 1974; 57: 920-925
    CrossrefPubMedGoogle Scholar
  • 35 Weiss HJ, Turitto VT, Baumgartner HR. Platelet adhesion and thrombus formation on subendothelium in platelets deficient in glycoproteins IIb-IIIa, Ib and storage granules. Blood 1986; 67: 322-330
    PubMedGoogle Scholar