Summary
Comparative analysis of high molecular weight kininogen (HK) in various commercial
congenital and immunodepleted deficiency plasmas was performed by immunoblotting of
HK. It was found, that some artificially depleted deficiency plasmas contained proteolytically
cleaved, kinin-free kininogen. In contrast, in all congenitally deficient plasmas,
HK was present in the intact, single chain form. Thus, cleavage of kininogen could
have been triggered by or during the immunodepletion procedure. It was seen, that
the degree of proteolytic cleavage and degradation of HK in depleted plasmas differed
among various manufacturers. E.g. depleted products of one company contained only
trace amounts of cleaved HK, in contrast to products of another one, in which HK was
completely degraded. The immunoblot analysis of HK reflects the occurrence of proteolytic
events during the production of artificially deficient plasmas and can therefore serve
as a quality control method.
Keywords
High molecular weight kininogen - Commercial, immunodepleted deficiency plasmas -
Proteolytic events during immunodepletion of plasmas
