Summary
Concentrations of sodium chloride up to 3 M increase the time necessary for the clot
formation from Limulus amebocyte lysate (LAL) induced with endotoxin. Sodium chloride at a concentration
of 4 M prevents clot formation by either precipitation or denaturation of procoagulase.
The time necessary for the activation of procoagulase by endotoxin is increased by
a change in the sodium chloride concentration from 0.15 M to 0.588 M. No effect on
the proteolytic phase or the polymerization phase of the clotting reaction is detected
by the increase in sodium chloride concentration from 0.15 M to 0.588 M. The authors
conclude that increased sodium chloride concentrations may aid the isolation of procoagulase.
