Summary
Antiserum to the carboxymethylated Aα chain of human fibrinogen has been produced
in rabbits and its reactions investigated in a double diffusion system. The antiserum
reacts with the carboxymethylated Aα chains, but not with carboxymethylatedγ chains,
nor with carboxymethylated Bβ chains, provided that the antiserum had been absorbed
with carboxymethylated Bβ chains.
The antiserum also reacts with Aα chains in the whole fibrinogen molecule, either
in plasma or in the purified form, and spur formation was observed when intact fibrinogen
was compared with high solubility fibrinogen, which contained little or no intact
Aα chain.