Summary
A convenient, accurate and reproducible high pressure liquid chromatographic method
for the quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma is described. The method involves the determination of total ether
extractable radioactivity, and a chromatographic separation to determine the relative
quantities of radio-labelled vitamin K1 and vitamin K1 epoxide. The method is useful over a wide range of ratios of the two compounds, and
has a coefficient of variation of approximately 5%.
