Download PDF
Thromb Haemost 1978; 39(02): 466-473
DOI: 10.1055/s-0038-1646706
Original Article
Schattauer GmbH Stuttgart

Quantitation of Radio-Labelled Vitamin K1 and Vitamin K1 Epoxide in Plasma by High Pressure Liquid Chromatography

Thorir D Bjornsson
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
,
Sarah E Swezey
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
,
Peter J Meffin
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
,
Terrence F Blaschke
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
› Author Affiliations
Further Information

Publication History

Received 21 July 1977

Accepted 20 October 1977

Publication Date:
12 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

A convenient, accurate and reproducible high pressure liquid chromatographic method for the quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma is described. The method involves the determination of total ether extractable radioactivity, and a chromatographic separation to determine the relative quantities of radio-labelled vitamin K1 and vitamin K1 epoxide. The method is useful over a wide range of ratios of the two compounds, and has a coefficient of variation of approximately 5%.

 

  • References

  • 1 Bell RG, Caldwell PT. 1973; Mechanism of warfarin resistance. Warfarin and the metabolism of vitamin K1. Biochemistry 12: 1759
    PubMedGoogle Scholar
  • 2 Bligh EG, Dyer WJ. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37: 911
    CrossrefPubMedGoogle Scholar
  • 3 Matschiner JT, Bell RG, Amelotti JM, Knauer TE. 1970; Isolation and characterization of a new metabolite of phylloquinone in the rat. Biochimica et biophysica acta 201: 309
    CrossrefPubMedGoogle Scholar
  • 4 Mayer H, Isler D. 1971. Vitamin K Group. II. Chemistry. In: Sebrell WH, Harris RS. (eds.) The Vitamins. Chemistry, Physiology, Pathology, Methods. Volume III. Academic Press; New York and London: 418
    Google Scholar
  • 5 Shearer MJ, Barkhan P, Webster GR. 1970; Absorption and excretion of an oral dose of tritiated vitamin K1 in man. British Journal of Haematology 18: 297
    CrossrefPubMedGoogle Scholar
  • 6 Shearer MJ, Mcburney A, Barkhan P. 1973; Effect of warfarin anticoagulation on vitamin metabolism in man. British Journal of Haematology 24: 471
    CrossrefPubMedGoogle Scholar
  • 7 Tishler M, Fieser LF, Wendler NL. 1940; Hydro, oxido and other derivatives of vitamin K1 and related compounds. Journal of the American Chemical Society 62: 2866
    CrossrefPubMedGoogle Scholar