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Thromb Haemost 1989; 61(03): 402-408
DOI: 10.1055/s-0038-1646604
Original Article
Schattauer GmbH Stuttgart

The Effect of Vascular Cell Seeding on Platelet Deposition in an In Vitro Capillary Perfusion Model

A Dekker
The University of Twente, Department of Chemical Technology, Enschede, The Netherlands
,
A Poot
The University of Twente, Department of Chemical Technology, Enschede, The Netherlands
,
T Beugeling
The University of Twente, Department of Chemical Technology, Enschede, The Netherlands
,
A Bantjes
The University of Twente, Department of Chemical Technology, Enschede, The Netherlands
,
W G van Aken
The University of Twente, Department of Chemical Technology, Enschede, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 11 November 1988

Accepted after revision 22 February 1989

Publication Date:
24 July 2018 (online)

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Summary

In view of the reported beneficial effects of cell seeding on the performance of synthetic vascular grafts, we determined the deposition of 111Indium-labelled human platelets in polyethylene capillary tubes covered with human endothelial cells (HEC) or smooth muscle cells (SMC) using an in vitro perfusion model. Platelet deposition decreased with increasing vascular cell coverage and was virtually absent when the number of adherent vascular cells of both types exceeded 50,0001cm2. Platelet deposition increased with increasing she ar rate (300-900 s-1) only when surface coverage was less than 50,000 vascular cells/cm2.

Deposition of Ca2+-ionophore A 23187-activated platelets in capillary tubes completely covered with SMC was significantly higher compared to capillaries covered with a similar number of HEC.

When HEC-lined capillaries were treated with aspirin, only the deposition of activated platelets increased slightly, but significantly. This platelet reactivity was more evident when the endothelial lining was not confluent.

These results demonstrate that, although seeding of HEC and SMC may both prevent the deposition of non-activated platelets to surfaces, non-aspirin-treated confluent HEC linings offer the best protection against deposition of activated platelets.

Keywords

Cell seeding - Platelet deposition - Flow conditions
 

  • References

  • 1 Snyder RW, Botzko KM. Woven, knitted and externally supported Dacron vascular prostheses. In: Biologic and Synthetic Vascular Prostheses. Stanley JC, Burkel WE, Lindenauer SM, Bartlett RH, Turcotte JG. (eds). Grune and Stratton; New York: 1982. p 485
    Google Scholar
  • 2 Graham LM, Bergan JJ. Expanded polytetrafluoroethylene vascular grafts: clinical status and experimental observations. In: Biologic and Synthetic Vascular Prostheses. Stanley JC, Burkel WE, Lindenauer SM, Bartlett RH, Turcotte JG. (eds) Grune and Stratton; New York: 1982. p 563
    Google Scholar
  • 3 Köveker GB, Burkel WE, Graham LM, Wakefield TW, Stanley JC. Endothelial cell seeding of expanded polytetrafluoroethylene vena cava conduits: effects on luminal production of prostacyclin, platelet adherence and fibrinogen accumulation. J Vase Surg 1988; 7: 600
    CrossrefPubMedGoogle Scholar
  • 4 Herring MB. Endothelial seeding of blood flow surfaces. In: Vascular Grafting, Clinical Applications and Techniques. Wright CB. (ed) John Wright PSG Inc.; Boston: 1983. p 275
    Google Scholar
  • 5 Berger K, Sauvage LR, Rao AM, Wood SJ. Healing of arterial prostheses in man: its incompleteness. Ann Surg 1972; 175: 118
    CrossrefPubMedGoogle Scholar
  • 6 Sharefkin JB, Latker C, Smith M, Cruess D, Clagett GP, Rich NM. Early normalization of platelet survival by endothelial seeding of Dacron arterial prostheses in dogs. Surgery 1982; 92: 385
    PubMedGoogle Scholar
  • 7 Stanley JC, Burkel WE, Ford JW. Enhanced patency of small- diameter, externally supported Dacron iliofemoral grafts seeded with endothelial cells. Surgery 1982; 92: 994
    PubMedGoogle Scholar
  • 8 Ortenwall P, Wadenvik H, Kutti J, Risberg B. Reduction in deposition of indium III-labeled platelets after autologous endothelial cell seeding of Dacron aortic bifurcation grafts in humans: a preliminary report. J Vase Surg 1987; 6: 17
    CrossrefPubMedGoogle Scholar
  • 9 Herring MB, Gardner A, Glover J. Dacron femoral-popliteal bypass grafts seeded with mechanically derived endothelium: an update. ASAIO J 1985; 8: 74
    PubMedGoogle Scholar
  • 10 Czervionke RL, Hoak JC, Fry GL. Effect of aspirin on thrombin- induced adherence of platelets to cultured cells from the blood vessel wall. J Clin Invest 1978; 62: 847
    CrossrefPubMedGoogle Scholar
  • 11 Fry GL, Czervionke RL, Hoak JC, Smith JB, Haycraft DL. Platelet adherence to cultured vascular cells: influence of prostacyclin. Blood 1980; 55: 271
    PubMedGoogle Scholar
  • 12 Moncada S, Palmer RM J, Higgs EA. Prostacyclin and endothelium- derived relaxing factor: biological interactions and significance. In: Thrombosis and Haemostasis 1987.. Verstraete M, Vermylen J, Lijnen HR, Amout J. (eds) International Society on Thrombosis and Haemostasis and Leuven University Press; Leuven: 1987. p 597
    Google Scholar
  • 13 Frangos JA, Eskin SG, Mclntire LV, Ives CL. Row effects on prostacyclin production by cultured human endothelial cells. Science 1985; 227: 1477
    CrossrefPubMedGoogle Scholar
  • 14 Rubanyi GM, Romero JC, Vanhoutte PM. Flow-induced release of endothelium-derived relaxing factor. Am J Physiol 1986; 250 H 1145
    PubMedGoogle Scholar
  • 15 Poot A, Beugeling T, Cazenave JP, Bantjes A, van Aken WG. Platelet deposition in a capillary perfusion model: quantitative and morphological aspects. Biomaterials 1988; 9: 126
    CrossrefPubMedGoogle Scholar
  • 16 Willems Ch, Astaldi GC B, de Groot PhG, Jansen MC, Gonsalves MD, Zeijlemaker WP, van Mourik JA, van Aken WG. Media conditioned by cultured vascular endothelial cells inhibit the growth of vascular smooth muscle cells. Exp Cell Res 1982; 139: 191
    CrossrefPubMedGoogle Scholar
  • 17 Loesberg C, van Wijk R, Zandbergen J, van Aken WG, van Mourik JA, de Groot PhG. Cell cycle-dependent inhibition of human vascular smooth muscle cell proliferation by PGE1 . Exp Cell Res 1985; 160: 117
    CrossrefPubMedGoogle Scholar
  • 18 Van Wachem PB, Reinders JH, van Buul-Wortelboer MF, de Groot PhG, van Aken WG, van Mourik JA. Von Willebrand factor in cultured human vascular endothelial cells from adult and umbilical cord arteries and veins. Thromb Haemostas 1986; 56: 189
    PubMedGoogle Scholar
  • 19 Cazenave JP, Hemmendinger S, Beretz A, Sutter-Bay A, Launay J. L’aggregation plaquettaire: outil d’investigation clinique et d’etude pharmacologique. Methodologie. Ann Biol Clin 1983; 41: 167
    PubMedGoogle Scholar
  • 20 Charo IF, Fitzgerald LA, Steiner B, Rail SC, Bekeart LS, Phillips DR. Platelet glycoproteins IIb and IIIa: evidence for a family of immunologically and structurally related glycoproteins in mammalian cells. Proc Natl Acad Sci USA 1986; 83: 8351
    CrossrefPubMedGoogle Scholar
  • 21 Newman PJ, Kawai Y, Montgomery RR, Kunicki TJ. Synthesis by cultured human umbilical vein endothelial cells of two proteins structurally and immunologically related to platelet membrane glycoproteins IIb and IIIa. J Cell Biol 1986; 103: 81
    CrossrefPubMedGoogle Scholar
  • 22 Charo IF, Bekeart LS, Phillips DR. Platelet glycoprotein IIb/IIIa- like proteins mediate endothelial cell attachment to adhesive proteins and the extracellular matrix. J Biol Chem 1987; 262: 9935
    PubMedGoogle Scholar
  • 23 Cheresh DA. Human endothelial cells synthesize and express an Arg-Gly-Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor. Proc Natl Acad Sci USA 1987; 84: 6471
    CrossrefPubMedGoogle Scholar
  • 24 Dejana E, Languino LR, Colella S, Corbascio GC, Plow E, Ginsberg M, Marchisio PC. The localization of a platelet GP IIb/IIIa-related protein in endothelial cell adhesion structures. Blood 1988; 71: 566
    PubMedGoogle Scholar
  • 25 Weiss HJ, Turitto VT. Prostacyclin (prostaglandin I2, PGI2) inhibits platelet adhesion and thrombus formation on subendothelium. Blood 1979; 53: 244
    PubMedGoogle Scholar
  • 26 Turitto VT, Baumgartner HR. Platelet surface interaction. In: Thrombosis and Haemostasis: Basic Principles and Clinical Practice. Colman RW, Hirsh J, Marder VJ, Salzman EW. (Eds) JB Lippincott Co.; Philadelphia: 1982. p 364
    Google Scholar
  • 27 Turitto VT, Benis AM, Leonard EF. Platelet diffusion in flowing blood. Indust Eng Chem Fund 1972; 11: 216
    PubMedGoogle Scholar
  • 28 Goldsmith HL. Red cell motions and wall interactions in tube flow. Fed Proc 1971; 30: 1641
    PubMedGoogle Scholar
  • 29 Goldsmith HL. The flow of model particles and blood cells and its relation to thrombogenesis. In: Progress in Hemostasis and Thrombosis. Spaet TH. (ed) Grune and Stratton Inc.; New York: 1972. p 97
    Google Scholar
  • 30 Turitto VT, Weis HJ, Baumgartner HR. Rheological factors influencing platelet interaction with vessel surfaces. J Rheol 1979; 23: 735
    CrossrefPubMedGoogle Scholar
  • 31 Eldor A, Vlodavsky I, Fuks Z, Muller TH, Eisert WG. Different effects of aspirin, dipyridamole and UD-CG 115 on platelet activation in a model of vascular injury: studies with extracellular matrix covered with endothelial cells. Thromb Haemostas 1986; 56: 333
    PubMedGoogle Scholar
  • 32 Marcus AJ, Broekman MJ, Weksler BB, Jaffe EA, Safier LB, Ullman HL, Tack-Goldman K. Interactions between stimulated platelets and endothelial cells in vitro. Philos Trans R Soc London 1981; 294: 343
    CrossrefPubMedGoogle Scholar
  • 33 Chesterman CN, Owe-Young R, Macpherson J, Krilis SA. Substrate for endothelial prostacyclin production in the presence of platelets exposed to collagen is derived from the platelets rather than the endothelium. Blood 1986; 67: 1744
    PubMedGoogle Scholar
  • 34 Baenziger NL, Becherer PR, Majerus PW. Characterization of prostacyclin synthesis in cultured human arterial smooth muscle cells, venous endothelial cells and skin fibroblasts. Cell 1979; 16: 967
    CrossrefPubMedGoogle Scholar
  • 35 Sneddon JM, Vane JR. Endothelium-derived relaxing factor reduces platelet adhesion to bovine endothelial cells. Proc Natl Acad Sci USA 1988; 85: 2800
    CrossrefPubMedGoogle Scholar
  • 36 Yue X, van der Lei B, Schakenraad JM, van Oene GH, Kuit JH, Feijen J, Wildevuur Ch RH. Smooth muscle cell seeding in biodegradable grafts in rats: a new method to enhance the process of arterial wall regeneration. Surgery 1988; 103: 206
    PubMedGoogle Scholar