Thromb Haemost 1988; 59(02): 248-250
DOI: 10.1055/s-0038-1642763
Original Articles
Schattauer GmbH Stuttgart

Epidermal Growth Factor Stimulates Prostacyclin Production by Cultured Human Vascular Endothelial Cells

Ari Ristimäki
1   Children's Hospital, University of Helsinki, Helsinki, Finland
,
Olavi Ylikorkala
2   The Department of Obstetrics and Gynecology, University of Helsinki, Helsinki, Finland
,
Jaakko Perheentupa
1   Children's Hospital, University of Helsinki, Helsinki, Finland
,
Lasse Viinikka
1   Children's Hospital, University of Helsinki, Helsinki, Finland
› Author Affiliations
Further Information

Publication History

Received 04 August 1987

Accepted after revision 03 December 1987

Publication Date:
21 May 2018 (online)

Preview

Summary

Epidermal growth factor (EGF) stimulated prostacyclin (PGI2) production by cultured human umbilical vein endothelial cells, as measured by radioimmunoassay of its stable metabolite 6-keto- prostaglandin F. This effect of EGF was dose-dependent, the lowest stimulatory concentration of EGF was 1.0 ng/ml and 100 ng/ml caused a 2.7 ± 0.3 (mean ± SEM) fold increase in the PGI2 synthesis. The stimulation appeared at 3-6 h of incubation and lasted at least 24 h. It was suppressed by EGF antibodies and blocked by protein synthesis inhibitor cycloheximide. Cells preincubated 12 h with EGF released also higher amounts of PGI2when incubated with thrombin for 5 min. It is concluded that EGF liberated from platelets during aggregation may prevent local thrombogenesis and atherogenesis by stimulating the release of the antiaggregatory, vasodilatory PGI2 from vascular endothelial cells.