Diabetologie und Stoffwechsel 2018; 13(S 01): S20
DOI: 10.1055/s-0038-1641812
Poster
Typ-2-Diabetes – Pathophysiologie I
Georg Thieme Verlag KG Stuttgart · New York

Oleuropein ameliorates palmitate-induced insulin resistance by increasing Glut4 translocation through activation of AMPK

H Alkhateeb
1   Yarmouk University, Basic Medical Sciences, Irbid, Jordan
› Author Affiliations
Further Information

Publication History

Publication Date:
26 April 2018 (online)

 

Question:

Oleuropein, the main constituent of leaves and fruits of the olive tree, has been found to be harmful to the normal homeostatic mechanisms of glucose regulation in rat skeletal muscle. Oleuropein ameliorates insulin resistance in skeletal muscle.

Methodology:

To examine this question, insulin resistance was rapidly induced by incubating (12h) soleus muscle with a high concentration of palmitate (2mM). Subsequently, we attempted to restore insulin sensitivity by incubating (12h) muscles with oleuropein (1.5mM), while maintaining high concentrations of palmitate.

Results:

Palmitate treatment for 12h reduced insulin-stimulated glucose transport, GLUT4 translocation, and AS160 phosphorylation. Oleuropein treatment (12h) fully restored insulin-stimulated glucose transport, GLUT4 translocation, and AS160 phosphorylation. Inhibition of PI3K phosphorylation with wortmannin (1µM) did not affect the oleuropein-induced improvements in insulin-stimulated glucose transport, GLUT4 translocation, and AS160 phosphorylation.

Conclusions:

These results suggested that the PI6K pathway does not account for activating the PI3K pathway. Taken altogether, it appears that oleuropein, through activation of another pathway like AMPK, may provide a possible strategy by which it amitates palmitate-induced insulin resistance in skeletal muscles.