Canine keratinocyte culture and use of a cultured epidermal autograft in a dog

D. M. Anderson; M. A. Stanley; R. A. S. White

doi:10.1055/s-0038-1632781

Veterinary and Comparative Orthopaedics and Traumatology, Inhaltsverzeichnis

Vet Comp Orthop Traumatol 2003; 16(04): 255-259
DOI: 10.1055/s-0038-1632781

Original Research

Schattauer GmbH

Canine keratinocyte culture and use of a cultured epidermal autograft in a dog

D. M. Anderson

¹Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

,

M. A. Stanley

²Epithelial Pathology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge

,

R. A. S. White

¹Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

› Institutsangaben

Abstract

Summary

A seven month old female Yorkshire Terrier was admitted with severe contracture and scarring of the ventral abdomen, thorax and limbs, after a scald injury. Surgical management consisted of multiple releasing and reconstruction skin flaps. Keratinocytes, harvested from a 1 cm² full thickness skin biopsy, were cultured on a lawn of irradiated feeder cells, and used as a cultured epithelial autograft on the remaining skin deficits. Epithelialisation of the deficits was complete at the first dressing change, five days after application. This is the first report of successful culture of canine keratinocytes suitable for use in skin reconstruction in a clinical case.

Keywords

Keratinocyte - graft - burn

Volltext

Referenzen

References
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