Download PDF
Nuklearmedizin 1970; 09(04): 341-349
DOI: 10.1055/s-0038-1624723
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Simple Method for Determining the Concentration of Serum Thyroxine by Competitive Displacement Analysis

Une méthode simple pour déterminer la concentration du sérum thyroxine par l’analyse du déplacement compétitifEine einfache Methode für die Bestimmung der Serum-Thyroxin-Konzentration durch kompetitive Verdrängungsanalyse
Csaba Bános
1   Radioisotope Department, János Hospital, Budapest (Director: Dr. József Takó) and Institute of Biophysics, Semmelweis University Medical School, Budapest (Director: Prof. Dr. Imre Tarján)
,
József Takó
1   Radioisotope Department, János Hospital, Budapest (Director: Dr. József Takó) and Institute of Biophysics, Semmelweis University Medical School, Budapest (Director: Prof. Dr. Imre Tarján)
,
Sándor Györgyi
1   Radioisotope Department, János Hospital, Budapest (Director: Dr. József Takó) and Institute of Biophysics, Semmelweis University Medical School, Budapest (Director: Prof. Dr. Imre Tarján)
,
Helen Sztraka
1   Radioisotope Department, János Hospital, Budapest (Director: Dr. József Takó) and Institute of Biophysics, Semmelweis University Medical School, Budapest (Director: Prof. Dr. Imre Tarján)
,
Istvan Kapus
1   Radioisotope Department, János Hospital, Budapest (Director: Dr. József Takó) and Institute of Biophysics, Semmelweis University Medical School, Budapest (Director: Prof. Dr. Imre Tarján)
› Author Affiliations
Further Information

Publication History

Received: 31 July 1970

Publication Date:
23 January 2018 (online)

Also available at
    Permissions and Reprints

Summary

A simplified method for the determination of serum thyroxine is described. The mathematical formulation of the standard curve required for the competitive displacement analysis permits an evaluation of the serum thyroxine concentration with the use of two standard values instead of measuring the full curve. The values calculated from the formula show a very close correlation with those obtained by comparison with the standard curve.

Une méthode simplifiée pour la détermination du sérum thyroxine est décrite. La formulation mathématique de la courbe standard, nécessaire à l’analyse du déplacement compétitif permet à calculer la concéntration du sérum thyroxine de deux valeurs standard sans la calibration entière de la courbe. Les concentrations calculées par la formule montrent une corrélation étroite avec ceux, évaluées relativement à la courbe calibrée.

Es wird eine einfache Methode für die Bestimmung von Serum-Thyroxin beschrieben. Die mathematische Formulierung der zur kompetitiven Verdrängungsanalyse nötigen Standardkurve ermöglicht die Berechnung der Serum-Thyroxin-Konzentration aus zwei Standardwerten, ohne die ganze Kurve messen zu müssen. Die sich aus der Formel ergebenden Werte zeigen eine enge Korrelation mit denjenigen, die sich relativ zu der Standardkurve ermitteln lassen.

 

  • References

  • 1 Banos Cs, Tako J, Rado J. P, Krasznai I, Göschl I. Diagnostic value of the 131I-labelled triiodothyronine red blood cell uptake test. Nucl.-Med 1967; 6: 124.
    Search in Google Scholar
  • 2 Barker S. B. Determination of protein-bound iodine. J. biol. Chem 1948; 173: 715.
    PubMedSearch in Google Scholar
  • 3 Dalrympe D. E, Utiger R. D. A micromethod for the determination of serum thyroxine: Studies of serum thyroxine concentration and thyroxine binding in rat serum. J. Lab. Clin. Med 1970; 75: 325.
    PubMedSearch in Google Scholar
  • 4 Ekins R. P. The estimation of thyroxine in human plasma by an electrophoretic technique. Clin. Chim. Acta 1960; 5: 453.
    CrossrefPubMedSearch in Google Scholar
  • 5 Kennedy J. A, Abelson D. M. Determination of serum thyroxine using a resin sponge technique. J. clin. Path 1967; 20: 89.
    CrossrefPubMedSearch in Google Scholar
  • 6 Lucis O. J, Cummings G. T, Matthews S, Burry C. Laboratory observations of assays of serum thyroxine and protein-bound iodine. J. Nucl. Med 1969; 10: 160.
    PubMedSearch in Google Scholar
  • 7 Maclagan N. F, Howorth P. J. N. Thyroid function studies using resin uptake of radioactive thyronines from serum and total thyroxine assay; the free thyroxine index. Clin. Sei 1969; 37: 45.
    Search in Google Scholar
  • 8 Murphy B. E. P, Pattee C. J. Determination of thyroxine utilizing the property of protein-binding. J. Clin. Endocr 1964; 24: 187.
    CrossrefPubMedSearch in Google Scholar
  • 9 Murphy B. E. P. The determination of thyroxine by competitive protein-binding analysis employing an anion exchange resin and radiothyroxine. J. Lab. Clin. Med 1965; 66: 161.
    Search in Google Scholar
  • 10 Murphy B. E. P, Pattee C. J. Clinical evaluation of a new method for the determination of serum thyroxine. J. Clin. Endocr 1966; 26 (02) 47.
    Search in Google Scholar
  • 11 Nakajima H, Kuramochi M, Horiguchi T, Kubo S. A new and simple method for the determination of thyroxine in serum. J. Clin. Endocr 1963; 26: 99.
    Search in Google Scholar
  • 12 Siersbaek-Nielsen K. Determination of serum thyroxine. Acta Med. Scand. 181 fasc 1967; 3: 327.
    Search in Google Scholar
  • 13 Sparagana M, Phillips G, Kucera L. Serum thyroxine by competitive protein binding analysis: clinical, statistical and comparative evaluation. J. Clin. Endocr 1969; 29: 191.
    CrossrefPubMedSearch in Google Scholar