Evaluation of a Simpler Technique for In-Vivo Labelling of Red Blood Cells with ^99mTc

The efficiency of labelling red blood cells with intravenously administered ^99mTc04, by prior oral administration of stannous chloride dihydrate, has been evaluated. Optimum labelling occured 1 hr after the oral dose of 200 mg of SnCl₂ · 2 H₂0 and 20 min after the intravenous injection of ^99mTc04. The mechanism of labelling has been shown to be diffusion of ^99mTc through the cell membrane followed by binding to haemoglobin in the presence of reducing agent. The in-vivo labelling technique has been found to be useful for the diagnosis of an abdominal mass and for static perfusion studies.

Es wurde die Effizienz der Markierung von Erythrozyten mit intravenös verabreichtem ^99mTc04 nach vorheriger oraler Gabe von Zinnchloriddihydrat untersucht. Die optimale Markierung trat 1 Std. nach der oralen Gabe von 200 mg SnCl2 · 2 H₂0 und 20 Min. nach der intravenösen Injektion von ^99mTc0₄ ein. Es konnte gezeigt werden, daß der Markierungsmechanismus aus der Diffusion des ^99mTc durch die Zellmembran besteht, die von einer Bindung an Hämoglobin in Gegenwart des Reduktionsmittels gefolgt wird. Die in vivo-Markierungsmethode wurde als nützlich zur Diagnose abdominaler Massen und bei statischen Perfusionsuntersuchungen gefunden.

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