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Nuklearmedizin 1981; 20(02): 51-63
DOI: 10.1055/s-0037-1620717
Originalarbeiten – Original Articles
Schattauer GmbH

A Comparative Investigation of the Gel Chromatography Column Scanning Method for Quality Control of 99mTc-Methylenediphosphonate

Eine vergleichende Untersuchung des Gelchromatographie Säulen-Scanning für die Qualitätskontrolle von 99mTc-Methylendiphosphonat
L. Darte
1   From the Department of Radiation Physics, University of Lund, Lasarettet, Lund, Sweden
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Publication History

Received: 18 December 1980

Publication Date:
10 January 2018 (online)

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Gel chromatography column scanning (GCS) is the method of choice for quality control of 99mTc-MDP preparations. Using this method all the labelled components are obtained rapidly in one simple test procedure. The influence of various parameters such as gel type, column size, prehistory of column, equilibration, eluent, elution volume and flow rate upon the results have been investigated. Test results for 99mTc-MDP have been compared for several different GCS systems, a few TLC systems and column chromatography with fraction collection. The GCS technique, optimized for testing 99mTc-MDP preparations, has been applied in a few experiments in which very good reproducibility is required: Labelling kinetics and stability when stored at room temperature or in a refrigerator and influence of the 99mTc/(99mTc + 99Tc) atomic ratio and of the amount of radioactivity on the 99mTc-MDP labelling yield, covering parameter ranges of clinical interest, have been studied.

Gelchromatographie Säulen-Scanning (GCS) ist die Methode der Wahl für die Qualitätskontrolle von 99mTc-Methy-lendiphosphonat. Mit der GCS-Methode werden alle markierten Komponenten schnell in einem einzigen, einfachen Testverfahren erhalten. Die Beeinflussung der Ergebnisse durch verschiedene Parameter wie Gel-Type, Größe der Säule, Vorgeschichte der Säule, Gleichgewicht sowie Natur, Volumen und Geschwindigkeit des Elutionsmittels wurde untersucht. Prüfungsergebnisse von 99mTc-MDP für verschiedene GCS-Systeme, einige Systeme der Dünnschichtchromatographie und konventionelle Gelchromatographie mit Sammlung von Eluatfraktionen wurden verglichen. Die optimale GCS-Technik zur Prüfung von 99mTc-MDP-Präparaten wurde in einigen Experimenten angewendet, die sehr gute Reproduzierbarkeit erfordern: Kinetik der Markierung und Stabilität bei Zimmertemperatur und im Kühlschrank, Beeinflussung des 99mTc/(99mTc + 99Tc) atomaren Verhältnisses und Beeinflussung der Radioaktivitätsmenge auf die Markierungseffizienz für Parameterbereiche von klinischer Bedeutung.

 

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