Studies of the Chemical and Biological Properties of the Skeletal Imaging Agent ^99mTc-Methylene Diphosphonate

Studies of the chemical and biological properties as well as of the optimization of a ^99mTc-MDP kit for clinical application have been performed. During the preparation of a Sn-MDP complex for labelling with ^99mTc, the molar ratio of the reactants [MDP: Sn (II)], the concentration of the Sn-MDP complex and the pH of the ^99mTc-MDP solution have been varied and their effects on biodistribution examined. The radiochemical composition of the labelled solutions was checked by paper chromatography, thin-layer chromatography and gel-filtration. The results of clinical applications for bone and early kidney imaging are also reported.

Es wurden die chemischen und biologischen Eigenschaften des ^99mTc-MDP sowie seine Eignung für den klinischen Anwendungsbereich untersucht. Während der Herstellung des Sn-MDP-Komplexes für die Markierung mit ^99mTc wurden die molaren Verhältnisse der Reagenzien [MDP: Sn (II)], die Konzentration des Sn-MDP-Komplexes und der pH-Wert der ^99mTc-MDP-Lösung variiert und deren Einfluß auf die Bioverteilung untersucht. Die radiochemische Zusammensetzung der markierten Lösungen wurde durch Papierchromatographie, Dünnschichtchromatographie und Gelfiltration überprüft. Die Ergebnisse der klinischen Anwendung in der Knochenszintigraphie und der frühen Nierenszintigraphie werden dargelegt.

• References

• 1 Brody K. R, Hosain P, Spencer R. P, Hosain F, Wagner H. N. Tc-99m-imidodiphosphate: an improved bone scanning radiopharmaceutical. Brit. J. Radiol 49: 267-269 1976;
• 2 Castronovo F. P, Callahan R. J. New bone scanning agent: Tc-99m-labelled l-hydroxy-ethylidene-l,l’-disodium phosphonate. J. nucl. Med 13: 823-827 1972;
• 3 Cohen Y, Besnard M. A rapid method of radiochemical purity determination for ^99mTc radiopharmaceuticals. J. nucl. Med 22: 74 1981;
• 4 Darte L. A comparative investigation of GCS method for quality control of ^99mTc-MDP. Nucl. Med 20: 51-63 1981;
• 5 Francis M. D, Ferguson D. L, Tofe A. J, Bevan J. A, Michaels S. E. Comparative evaluation of three diphospho-nates: in vitro osteogenic uptake. J. nucl. Med 21: 1185-1189 1980;
• 6 Glass E, de Nardo G, Hines H. Immediate renal imaging and renography with ^99mTc-MDP to assess renal blood flow, excretory function, and anatomy. Radiology 135: 187-190 1980;
• 7 Hosain P. ^99mTc-Pyrophosphate: a simple and reproducible bone scanning agent. Brit. J. Radiol 46: 724-728 1973;
• 8 Johannsen B, Spies H. Technetium-Verbindungen. Zentralinstitut für Kernforschung. 47 Rossendorf, GDR; 1981
• 9 Nelson M. F, Melton R. E, van Wazer J. R. Sodium trimetaphosphate as a bone imaging agent. J. nucl. Med 16: 1043-1048 1975;
• 10 Owunwanne A, Weber D, O’Mara R, Wilson G. Factors influencing the radiochemical analysis of ^99mTc radiopharmaceuticals by paper chromatography. J. label. Comp, and Radiopharm 13: 261-262 1977;
• 11 Perez R, Cohen Y, Henry R. A new radiopharmaceutical for ^99mTc bone scanning. J. nucl. Med 13: 788-789 1972;
• 12 Rudd T, Allen D, Hartnett D. ^99mTc-Methylene diphosphonate: biologic and clinical comparison. J. nucl. Med 18: 872-876 1977;
• 13 Schwarz A, Kloss G. ^99mTc-DPD - a new skeletal imaging agent. J. nucl. Med 22: 77 1981;
• 14 Subramanian G, McAfee J. G. A new complex of ^99mTc for skeletal imaging. Radiology 99: 192-196 1971;
• 15 Subramanian G, McAfee J. G, Bell E. G. ^99mTc-polyphosphate as a skeletal imaging agent. Radiology 102: 701-704 1972;
• 16 Subramanian G, Blair R. J, Kallfelz F. A. ^99mTc-MDP: a superior agent for skeletal imaging. J. nucl. Med 14: 640 1973;
• 17 Subramanian G, McAfee J. G, Blair R. J, Kallfelz F. A, Thomas F. D. ^99mTc-MDP - a superior agent for skeletal imaging: comparison with other Tc complexes. J. nucl. Med 16: 744-755 1975;
• 18 Wang T, Hosain P, Spencer R. P, Ahlquist K, Hosain F. Synthesis, radiotechnetium labelling and comparison of biologic behavior of longer-chain analogs of methylene diphosphonate. J. nucl. Med 19: 1151-1153 1978;
• 19 Wang T, Fawwaz R, Johnson L, Mojdehi G, Johnson P. Bone-seeking properties of ^99mTc carbonyl diphosphonic acid, dihydroxy methylene diphosphonic acid and monohy-droxy methylene phosphonic acid. J. nucl. Med 21: 767-770 1980;
• 20 Yano Y, McRae J, can Dyke D. C. ^99mTc-labelled stannous ethane-1-hydroxy-1, 1’-diphosphonate: a new bone scanning agent. J. nucl. Med 14: 73-78 1973;