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Thromb Haemost 2001; 86(06): 1421-1424
DOI: 10.1055/s-0037-1616745
Review Article
Schattauer GmbH

Hepatic Thrombopoietin mRNA Is Increased in Acute Inflammation

Eva-Maria Wolber
1   Institute of Physiology, Medical University of Luebeck, Luebeck, Germany
,
Joachim Fandrey
2   Institute of Physiology, University of Essen, Essen, Germany
,
Urszula Frackowski
1   Institute of Physiology, Medical University of Luebeck, Luebeck, Germany
,
Wolfgang Jelkmann
1   Institute of Physiology, Medical University of Luebeck, Luebeck, Germany
› Author Affiliations
Further Information

Publication History

Received 03 January 2001

Accepted after resubmission 04 September 2001

Publication Date:
12 December 2017 (online)

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Summary

The plasma concentration of thrombopoietin (TPO) in general is inversely related to the mass of platelets and megakaryocytes. However, reactive thrombocytosis of inflammatory disease is accompanied by elevated TPO levels. To investigate whether the rate of TPO mRNA expression is altered during acute inflammation, rats were injected with bacterial lipopolysaccharide (LPS). After 6 h, total RNA from liver and kidney was reverse transcribed and analyzed by competitive PCR for TPO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). LPS-treated rats showed a significant increase in hepatic TPO mRNA concentration. The ratio of TPO to GAPDH mRNA was 3.5 ± 0.6% in the livers of control rats and 8.3 ± 2.0% in the livers of LPS-treated rats (mean ± SD). Thus, reactive thrombocytosis of inflammatory disease might result from an increase in hepatic TPO production. Since platelets are involved in the immune reaction, reactive thrombocytosis may be a mechanism of host defense.

Keywords

Thrombopoietin - inflammation - reactive thrombocytosis - acute phase reaction - LPS
 

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