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Vet Comp Orthop Traumatol 2007; 20(01): 12-17
DOI: 10.1055/s-0037-1616581
Original Research
Schattauer GmbH

Reactive changes in the adolescent porcine spine with disc degeneration due to endplate injury

S. Holm
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
,
A. Baranto
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
,
A. Kaigle Holm
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
,
L. Ekström
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
,
L. Swärd
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
,
T. Hansson
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
,
H.-A. Hansson
1   Department of Orthopaedics, Sahlgrenska University Hospital, and Institute of Anatomy and Cell Biology, Göteborg University, Göteborg, Sweden
› Author Affiliations
Further Information

Publication History

Received 06 March 2006

Accepted 29 March 2006

Publication Date:
21 December 2017 (online)

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Summary

Degenerative and reactive structural alterations occurring after experimentally-induced disc degeneration were evaluated using a porcine model. A cranial perforation was made through the L4 vertebral endplate into the nucleus pulposus. Three months later, the lumbar intervertebral disc and adjacent vertebrae were dissected, fixed in formalin and further processed for histopathological analyses. The results showed that there were nucleus pulposus fragments, rather than a distinct border between the nucleus and annulus fibrosus. The central lamellae were distorted and delamination of the outer anterior layers was observed. Blood vessels emerged from the adjacent tissue, penetrated the annulus and branched into the residues of the nucleus. Nerve fibres accompanying the blood vessels could be recognized in the disc within the connective scar tissue. The epiphyseal cartilage plates in the vertebrae were hypertrophic in several areas and there was bone formation directed towards the centre of the vertebral body and the disc. Hypertrophic hyaline cartilage, newly formed bone and scar tissue filled the injury canal. A slight chronic inflammatory reaction was evident along vascular buds. The reactive changes dominated over the degenerated features in the operated disc. Physiological loading enhanced the infiltration of various tissue types characterizing immature cartilage formation. Prominent neovascularisation of the central parts of the disc is likely to be of key importance in turning the degenerative features of the remaining tissue into reactive healthy structures.

Keywords

Disc injury - osteogenesis - chondrogenesis - epiphyseal cartilage - fibrosis
 

  • References

  • 1 Holm Holm, Urban J. The intervertebral disc: factors contributing to its nutrition and matrix turnover. In: Helminen HJ, Kiviranta I, Saamanen AM. et al. (eds). Joint Loading - Biology and Health of Articular Structures. Bristol: John Wright and Sons Ltd; 1987: 187-226.
    Google Scholar
  • 2 Lotz JC, Colliou OK, Chin JR. et al. Compression- induced degeneration of the intervertebral disc: an in vivo mouse model and finite element study. Spine 1998; 23: 2493-2506.
    CrossrefPubMedGoogle Scholar
  • 3 Nachemson A. Future of Low Back Pain. In: Wiesel S.W, Weinstein J. et al. (eds). The Lumbar Spine. WB. Saunders; 1996: 28-42.
    Google Scholar
  • 4 Salminen JJ, Erkintalo-Tertti MO, Paajanen HE. Magnetic resonance imaging findings of lumbar spine in the young: correlation with leisure time physical activity, spinalmobility, andtrunkmuscle strength in 15-year-old pupils with or without low- back pain. J Spinal Disord 1993; 6: 386-391.
    CrossrefPubMedGoogle Scholar
  • 5 Holm S. Pathophysiology of disc degeneration. In Stromqvist B. (ed). The degenerative lumbar spine. Munksgaard; 1993: 13-15.
    Google Scholar
  • 6 Holm S. Nutrition and Pathophysiologic Aspects of the Lumbar Intervertebral Disc. In: Wiesel S.W, Weinstein J. et al. (eds) The Lumbar Spine. W.B. Saunders; 1996: 285-310.
    Google Scholar
  • 7 Kaigle Holm AM, Holm S. Biomechanical Considerations of Disc Degeneration. In: Herkowitz H N, Dvorak J. et al. (eds). The Lumbar Spine. Lippincott Williams & Wilkins; 2004: 31-45.
    Google Scholar
  • 8 Ayad S, Weiss JB. Biochemistry ofthe intervertebral disc. In: Jayson MIV. (ed). The lumbar spine and back pain. Churchill Livingstone; 1987: 100-137.
    Google Scholar
  • 9 Bradford DS, Cooper KM, Oegema TR. Chymo- papain, chemonucleolysis and nucleus pulposus regeneration. J Bone Joint Surg 1983; 65A: 1220-1231.
    PubMedGoogle Scholar
  • 10 Norcross JP, Lester GE, Weinhold P. et al. An in vivo model of degenerative disc disease. J Orthop Res 2003; 21: 183-188.
    CrossrefPubMedGoogle Scholar
  • 11 Iatridis JC, Mente PL, Stokes IA. et al. Compression-induced changes in intervertebral disc properties in a rat tail model. Spine 1999; 24: 996-1002.
    CrossrefPubMedGoogle Scholar
  • 12 Karlsson L, Lundin O, Ekstrom L. et al. Injuries in adolescent spine exposed to compressive loads: an experimental cadaveric study. J Spinal Dis 1998; 11: 501-507.
    PubMedGoogle Scholar
  • 13 Kroeber MW, Unglaub F, Wang H. et al. New in vivo animal model to create intervertebral discdegeneration and to investigate the effects of therapeutic strategies to stimulate disc regeneration. Spine 2002; 27: 2686-2690.
    PubMedGoogle Scholar
  • 14 Walsh A, Bradford DS, Kleinstueck F. et al. In situ growth factor stimulation of degenerated intervertebral discs. Proceedings from ISSLS. Adelaide, Australia: 2000
    Google Scholar
  • 15 Kaigle AM, Holm SH, Hansson TH. Kinematic behavior of the porcine lumbar spine - a chronic lesion model. Spine 1997; 22: 2796-2806.
    CrossrefPubMedGoogle Scholar
  • 16 Kaigle A, Ekstrom L, Holm S. et al. In vivo dynamic stiffness of the lumbar spine exposed to cyclic loading: influence of load and degeneration. J Spinal Dis 1998; 11: 65-70.
    PubMedGoogle Scholar
  • 17 Kaapa E. Collagens, proteoglycans, and neural structures in aporcine model of intervertebral disc degeneration Dissertation. 1993. Oulu, Finland:
    Google Scholar
  • 18 Latham JM, Pearcy MJ, Costi JJ. et al. Mechanical consequences of annular tears and subsequent intervertebral disc degeneration. Clin Biomech 1994; 9: 211-219.
    CrossrefPubMedGoogle Scholar
  • 19 Lipson Lipson, Muir H. Proteoglycans in experimental intervertebral disc degeneration. Spine 1981; 6: 194-210.
    CrossrefPubMedGoogle Scholar
  • 20 Osti OL, Vernon-Roberts B, Fraser RD. Annulus tears and intervertebral disc degeneration. An experimental study using an animal model. Spine 1990; 15: 762-767.
    PubMedGoogle Scholar
  • 21 Holm S, Kaigle Holm A, Ekstrom L. et al. Experimental Disc Degeneration Due to Endplate Injury. J Spinal Disord Tech 2004; 17 1 64-71.
    CrossrefPubMedGoogle Scholar
  • 22 Baranto A, Ekstrom L, Holm S. et al. Vertebral fractures and separations of endplates after traumatic loading of adolescent porcine spines with experimentally induced disc degeneration. Clin Biomech 2005; 20: 1046-1054.
    CrossrefPubMedGoogle Scholar
  • 23 Sward L, Hellstrom M, Jacobsson Betal. Disc degeneration and associated abnormalities of the spine in elite gymnasts. Amagnetic resonance imaging study Spine 1991; 16: 437-443.
    PubMedGoogle Scholar
  • 24 Sward L, Hellstrom M, Jacobsson B. et al. Back pain and radiologic changes in the thoraco-lumbar spine of athletes. Spine 1990; 15: 124-129.
    PubMedGoogle Scholar
  • 25 Lundin O, Ekstrom L, Hellstrom M. et al. Exposure of the porcine spine to mechanical compression: differences in injury pattern between adolescents and adults. Eur Spine J 2000; 9: 466-471.
    CrossrefPubMedGoogle Scholar
  • 26 Sward L, Hellstrom M, Jacobsson B. et al. Vertebral ring apophysis injury in athletes. Is the etiology different in the thoracic and lumbar spine? Am J Sports Med 1993; 21: 841-845.
    PubMedGoogle Scholar
  • 27 Adams P, Eyre DR, Muir H. Biochemical aspects of development and ageing of human lumbar in- tervertebral discs. Rheumatol Rehabil 1977; 16: 22-29.
    CrossrefPubMedGoogle Scholar
  • 28 Adams MA, Freeman BJC, Morrison HP. et al. Mechanical initiation of intervertebral disc degeneration. Spine 2000; 25: 1625-1636.
    CrossrefPubMedGoogle Scholar
  • 29 Lyons G, Eisenstein SM, Sweet MBE. Biochemical changes in intervertebral disc degeneration. Bio Biophysic Acta 1981; 673: 443-453.
    PubMedGoogle Scholar
  • 30 Jennische E, Skottner A, Hansson HA. Dynamic changes in insulin-like growth factor I immuno- reactive correlate to repair events in rat ear after freeze-thaw injury. Exp Mol Pathol 1987; 47: 193-201.
    CrossrefPubMedGoogle Scholar
  • 31 Tanaka M, Nakahara S, Inoue H. A pathologic study of discs in the elderly. Separation between the cartilaginous endplate and the vertebral body. Spine 1993; 18: 1456-1462.
    PubMedGoogle Scholar
  • 32 Vernon-Roberts B. Disc pathology and disease states. In: Ghosh P. (ed). The Biology of the Intervertebral Disc. Vol II. Boca Raton, FL, USA: CRC Press; 1988: 73-119.
    Google Scholar