Thromb Haemost 2001; 85(06): 1097-1103
DOI: 10.1055/s-0037-1615972
Review Article
Schattauer GmbH

The Effect of Platelet PlA Polymorphism on Experimental Thrombus Formation in Man Depends on Blood Flow and Thrombogenic Substrate

Authors

  • Yves Cadroy

    1   Laboratoire de Recherche sur l’Hémostase et la Thrombose, CHU Purpan, Toulouse, France
  • Kjell Sakariassen

    2   Department of Biology, Division of General Physiology, University of Oslo, Oslo, Norway
  • Hélène Grandjean

    3   INSERM, Hôpital La Grave, Toulouse
  • Claire Thalamas

    4   Centre d’Investigation Clinique, CHU Purpan, Toulouse, France
  • Bernard Boneu

    1   Laboratoire de Recherche sur l’Hémostase et la Thrombose, CHU Purpan, Toulouse, France
  • Pierre Sié

    1   Laboratoire de Recherche sur l’Hémostase et la Thrombose, CHU Purpan, Toulouse, France
Weitere Informationen

Publikationsverlauf

Received 13. November 2000

Accepted after resubmission 06. Februar 2001

Publikationsdatum:
12. Dezember 2017 (online)

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Summary

A number of studies have reported conflicting data on the association of the PlA1/PlA2 polymorphism of the GPIIIa gene and coronary syndromes. We have investigated the effect of this polymorphism on experimental platelet thrombus formation in man. Forty healthy male volunteers were genotyped for the PlA1/PlA2 polymorphism. Thrombus formation was induced ex vivo by exposing a tissue factor (TF) or a collagencoated coverslip in a parallel plate perfusion chamber to native blood for 2 and 4 min. The shear rates at these surfaces were 650 and 2,600 s–1. Platelet and fibrin deposition was quantified by immunoenzymatic methods. The frequencies of PlA1/PlA1 and PlA1/PlA2 genotypes were 52.5% and 47.5%, respectively. Ex vivo deposition of fibrin on TF was not affected by the PlA1/PlA2 polymorphism. However, the ex vivo platelet deposition at 650 s–1 was higher in blood from PlA1/PlA1 individuals than in PlA1/PlA2 individuals (P = 0.008 at 4 min). On collagen, neither fibrin nor platelet deposition was significantly affected by the PlA1/PlA2 polymorphism. Platelet thrombus formation is significantly influenced by genetic variations in the GPIIIa platelet receptor. This effect depends on the blood flow properties and the nature of the thrombogenic stimulus.