Factor VIII Arg2304 → His Binds to Phosphatidylserine and Is a Functional Cofactor in the Factor X-ase Complex

Deborah A. Lewis; Karen D. Moore; Thomas L. Ortel

doi:10.1055/s-0037-1615697

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(02): 260-264
DOI: 10.1055/s-0037-1615697

Review Article

Schattauer GmbH

Factor VIII Arg₂₃₀₄ → His Binds to Phosphatidylserine and Is a Functional Cofactor in the Factor X-ase Complex

Deborah A. Lewis

¹Department of Medicine, Division of Hematology, and the Department of Pathology, Duke University Medical Center, Durham, NC, USA

,

Karen D. Moore

¹Department of Medicine, Division of Hematology, and the Department of Pathology, Duke University Medical Center, Durham, NC, USA

,

Thomas L. Ortel

¹Department of Medicine, Division of Hematology, and the Department of Pathology, Duke University Medical Center, Durham, NC, USA

› Author Affiliations

Abstract

Summary

Four factor VIII light chain constructs containing hemophilia A mutations at R2304 and R2307 were prepared and expressed in mammalian cells. These mutations are located in a putative phosphatidylserine binding site identified by peptide studies (spanning amino acids 2303-2332). The levels of all four mutants in conditioned medium were significantly less than wild type by immunoprecipitation and ELISA. R2304H and wild type factor VIII light chains were concentrated by cation exchange chromatography from medium. R2304H and wild type factor VIII light chains bound immobilized phosphatidylserine similarly. The reconstituted cofactor activity of R2304H factor VIII light chain was slightly greater than wild type factor VIII light chain. These results are consistent with the recently reported crystal structure of factor VIII C2 domain that suggests R2304H is not directly involved in phospholipid binding. The observed clinical phenotype is probably due to decreased circulating levels of a functional protein.

Keywords

Factor VIII - factor VIII light chain - hemophilia - phospholipid binding

Full Text

References

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