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Thromb Haemost 2001; 85(02): 296-302
DOI: 10.1055/s-0037-1615683
Review Article
Schattauer GmbH

Vascular Endothelial Growth Factor Enhances the Expression of Urokinase Receptor in Human Endothelial Cells via Protein Kinase C Activation

Marielle E. Kroon
1   Gaubius Laboratory TNO-PG, Leiden, The Netherlands
,
Pieter Koolwijk
1   Gaubius Laboratory TNO-PG, Leiden, The Netherlands
,
Mario A. Vermeer
1   Gaubius Laboratory TNO-PG, Leiden, The Netherlands
,
Bea van der Vecht
1   Gaubius Laboratory TNO-PG, Leiden, The Netherlands
,
Victor W. M. van Hinsbergh
1   Gaubius Laboratory TNO-PG, Leiden, The Netherlands
2   Institute for Cardiovascular Research, Vrije Universiteit, Amsterdam, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 15 June 2000

Accepted after revision 08 September 2000

Publication Date:
08 December 2017 (online)

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Summary

Among other proteolytic enzymes, the urokinase-type plasminogen activator (u-PA)/plasmin cascade contributes to cell migration and the formation of capillary-like structures in a fibrinous exudate. The u-PA receptor (u-PAR) focuses proteolytical activity on the cell surface of the endothelial cell and hereby accelerates the pericellular matrix degradation. Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF)-2 enhance u-PA receptor expression in human endothelial cells. In this paper we show that the protein kinase C (PKC) inhibitors Ro31-8220 and GF109203X inhibit VEGF165-induced u-PAR antigen expression in human endothelial cells, whereas PKC inhibition had no effect on FGF-2-induced u-PAR antigen enhancement. In addition, inhibition of PKC activity had no effect on VEGF165-or FGF-2-induced proliferation in human endothelial cells. We conclude that VEGF165 induces u-PAR via a PKC-dependent pathway, whereas proliferation is induced via a different pathway probably involving tyrosine phosphorylation of proteins downstream of the VEGF receptors.

Keywords

u-PAR - VEGF - PKC - human endothelial cells
 

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