Detection of the 20210 A/G Variation in the Prothrombin Gene by Denaturing Gradient Gel Electrophoresis (DGGE)

Thromb Haemost 1998; 79(05): 1055-1056
DOI: 10.1055/s-0037-1615120

Letters to the Editor

Schattauer GmbH

Sylvie Patri

¹From the Laboratoire de Génétique Cellulaire et Moléculaire, CHU de Poitiers, France

,

Jean Claude Chomel

¹From the Laboratoire de Génétique Cellulaire et Moléculaire, CHU de Poitiers, France

,

Sandra Salmeron

¹From the Laboratoire de Génétique Cellulaire et Moléculaire, CHU de Poitiers, France

,

Catherine Boinot

²From the Laboratoire d’Hématologie et Maladies du Sang, CHU de Poitiers, France

,

Dominique Laneelle

³From the Laboratoire d’Hématologie et d’Immunologie, CH de Niort, France

,

Yvette Sultan

²From the Laboratoire d’Hématologie et Maladies du Sang, CHU de Poitiers, France

,

Alain Kitzis

¹From the Laboratoire de Génétique Cellulaire et Moléculaire, CHU de Poitiers, France

References
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2 Poort SR, Bertina RM, Vos HL. Rapid detection of the prothrombin 20210 A variation by allele specific PCR. Thromb Haemost 1997; 78: 1157-8.
3 Myers RM, Maniatis T, Lerman LS. Detection and localization of single base change by denaturing gradient gel electrophoresis. Methods Enzymol 1987; 155: 501-27.
4 Degen SJ, Davie EW. Nucleotide sequence of the gene for human prothrombin. Biochem 1987; 26: 6165-77.
5 Bancroft JD, Schaefer LA, Degen SJ. Characterization of the Alu-rich 5’-flanking region of the human prothrombin-encoding gene: identification of a positive cis-acting element that regulates liver-specific expression. Gene 1990; 95: 253-60.
6 Sheffield VC, Cox DR, Lerman LS, Myers RM. Attachment of a 40-base-pair G+C-rich sequence (GC clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci USA 1989; 86: 232-6.