Detection of the 20210 A/G Variation in the Prothrombin Gene by Denaturing Gradient Gel Electrophoresis (DGGE)

• References

• 1 Poort SR, Rosendaal FR, Reitsma PH, Bertina RM. A common genetic variation in the 3’-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood 1996; 88: 3698-703.
• 2 Poort SR, Bertina RM, Vos HL. Rapid detection of the prothrombin 20210 A variation by allele specific PCR. Thromb Haemost 1997; 78: 1157-8.
• 3 Myers RM, Maniatis T, Lerman LS. Detection and localization of single base change by denaturing gradient gel electrophoresis. Methods Enzymol 1987; 155: 501-27.
• 4 Degen SJ, Davie EW. Nucleotide sequence of the gene for human prothrombin. Biochem 1987; 26: 6165-77.
• 5 Bancroft JD, Schaefer LA, Degen SJ. Characterization of the Alu-rich 5’-flanking region of the human prothrombin-encoding gene: identification of a positive cis-acting element that regulates liver-specific expression. Gene 1990; 95: 253-60.
• 6 Sheffield VC, Cox DR, Lerman LS, Myers RM. Attachment of a 40-base-pair G+C-rich sequence (GC clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci USA 1989; 86: 232-6.