Evaluation of Thrombopoiesis in Thrombocytopenic Disorders by Simultaneous Measurement of Reticulated Platelets of Whole Blood and Serum Thrombopoietin Concentrations

Yukako Koike; Akiko Yoneyama; Jun Shirai; Tateru Ishida; Eriko Shoda; Kouji Miyazaki; Sinji Sunaga; Ryoichi Horie; Katunori Aoki; Kazuhiko Koike; Iturou Ogata; Tomoyuki Tahara; Takashi Kato; Kazuhiko Nakahara; Toshitugu Kariya; Masaaki Higashihara

doi:10.1055/s-0037-1615024

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1998; 79(06): 1106-1110
DOI: 10.1055/s-0037-1615024

Rapid Communication

Schattauer GmbH

Evaluation of Thrombopoiesis in Thrombocytopenic Disorders by Simultaneous Measurement of Reticulated Platelets of Whole Blood and Serum Thrombopoietin Concentrations

Yukako Koike

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Akiko Yoneyama

²Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan

,

Jun Shirai

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Tateru Ishida

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Eriko Shoda

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Kouji Miyazaki

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Sinji Sunaga

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Ryoichi Horie

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Katunori Aoki

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Kazuhiko Koike

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Iturou Ogata

¹From the First Department of Internal Medicine, Tokyo, Japan

,

Tomoyuki Tahara

⁴Pharmaceutical Reseach Laboratory, Kirin Brewery Co. Ltd., Maebashi, Japan

,

Takashi Kato

⁴Pharmaceutical Reseach Laboratory, Kirin Brewery Co. Ltd., Maebashi, Japan

,

Kazuhiko Nakahara

²Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan

,

Toshitugu Kariya

³Division of Haematology, Tokyo Kosei Nenkin Hospital, Tokyo, Japan

,

Masaaki Higashihara

⁵Fourth Department of Internal Medicine, Kitasato University, School of Medicine, Kanagawa, Japan

› Institutsangaben

Abstract

Summary

To evaluate thrombopoiesis in thrombocytopenic disorders, we simultaneously determined reticulated platelet counts in whole blood by FACScan flow cytometry and serum thrombopoietin (TPO) concentrations by a sensitive sandwich ELISA. The subjects were 40 healthy volunteers and 45 thrombocytopenic patients. In idiopathic thrombocytopenic purpura (ITP), the percentage of reticulated platelets was significantly elevated (5.61 ± 2.02%: mean ± SD) relative to normal controls (2.17 ± 0.90%), but serum TPO concentrations (1.91 ± 1.27 fmol/l) did not differ significantly from the normal range (1.43 ± 0.62 fmol/l). The patients with aplastic anemia (AA) had decreased reticulated platelet counts and markedly increased serum TPO concentrations (13.65 ± 10.64 fmol/l). In thrombocytopenic patients with liver cirrhosis (LC), the absolute number of reticulated platelets (1.65 ± 1.11 × 10⁹/l) decreased similarly that in AA. However, serum TPO concentrations (1.38 ± 0.50 fmol/l) did not increase in contrast to AA. Our findings suggested a possible dual mechanism of thrombocytopenia in LC; that is, thrombocytopenia in LC results from the decreased TPO production primarily in the liver adding to an increase in platelet sequestration in the spleen.

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