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Thromb Haemost 1999; 82(01): 93-99
DOI: 10.1055/s-0037-1614635
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Adsorption of Vitamin K-dependent Proteins to Live Cell Membranes Measured under Flow Conditions

Maria P. McGee
1   From the Department of Medicine, Medical School of Wake Forest University, Winston-Salem, USA
,
Hoa Teuschler
1   From the Department of Medicine, Medical School of Wake Forest University, Winston-Salem, USA
› Author AffiliationsThis work was supported by NSF grant MCB 9601411.
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Publication History

Received 08 December 1998

Accepted after revision 05 March 1999

Publication Date:
11 December 2017 (online)

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Summary

Mechanisms mediating initial adsorption of coagulation proteins to live cells were investigated. Adsorption kinetics were examined under varying flow conditions using tracer-dilution techniques in perfused spherical monolayers of cells expressing tissue factor. At biologically relevant time and concentration ranges, rates exceeded by 2-12 fold the theoretical maximum calculated for steady-state diffusion. Rates were correlated with aqueous-phase flux of reactants and were found to be largely independent of the density of reactive sites on the membrane. Average adsorption rate of factor VIIa at 4 ηM and flow velocity of 0.8 ηm s-1 was 5 × 107 s-1 cm-2. Adsorption rates of homologous coagulation factors IX and X under similar conditions were 5 and 9 × 107 s-1 cm-2. Results indicate that flow can effectively increase the rate of coagulation factor adsorption to the membrane of live cells. They also imply that factors affecting blood flow velocity and vessel permeability influence the rate of membrane-dependent coagulation reactions.

 

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