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Thromb Haemost 1999; 81(05): 727-732
DOI: 10.1055/s-0037-1614562
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Schattauer GmbH

Production of Recombinant Human Protein C In Vitro and In Vivo by Muscle Cells

Ataç Türkay
1   From the Departments of Human Genetics The University of Michigan Medical School, Ann Arbor, Michigan
2   Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan
3   Wayne State University School of Medicine, Department of Internal Medicine, Detroit, MI, USA
,
Jian-Min Wang
1   From the Departments of Human Genetics The University of Michigan Medical School, Ann Arbor, Michigan
,
Kotoku Kurachi
1   From the Departments of Human Genetics The University of Michigan Medical School, Ann Arbor, Michigan
› Author Affiliations
Further Information

Publication History

Received 30 September 1998

Accepted after revision 28 January 1999

Publication Date:
09 December 2017 (online)

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Summary

Protein C plays a key role in a natural anticoagulation mechanism, and is also implicated in fibrinolytic and anti-inflammatory functions. Here we describe the production of biologically active human protein C by muscle-targeted gene transfer. Human protein C expression vectors were designed and constructed to produce human protein C in skeletal muscle cells. These vectors were tested in transient and stable transfections of SCID mice myoblasts. Stably transfected cells produced as high as 2.27 μg/106 cells/day. Human protein C produced had a relative activity of 92 ± 8% compared to the plasma derived human protein C, and was composed of α and β forms, 69% and 31%, respectively. After implantation of stably transfected myoblasts into the hind limb muscles of SCID mice, systemic stable production of human protein C in a range of 31-116 ng/ml serum was obtained up to at least 2.5 months.

Current address: J. Wang, Department of Hematology, Changai Hospital, 174 Changai Road, Yangpu District, Shangai 200433, China

 

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