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DOI: 10.1055/s-0037-1614453
dRVVT Is more Sensitive than KCT or TTI for Detecting Lupus Anticoagulant Activity of Anti-β2-glycoprotein I Autoantibodies
Publikationsverlauf
Received17. November 1997
Accepted after resubmission22. Oktober 1998
Publikationsdatum:
08. Dezember 2017 (online)
Summary
Anti-β2-glycoprotein I (β2-GPI) antibodies behave as classical Lupus Anticoagulants (LA), as they inhibit phospholipid-dependent coagulation reactions and their activity disappears in the presence of excess exogenous phospholipids (PLs). We have recently shown that a certain amount of PLs in the dilute Russell Viper Venom Time (dRVVT) test system is required to express LA activity of anti β2-GPI antibodies. We have now extended this observation to two other tests, i.e., Kaolin Clotting Time (KCT) in which PLs are not added, and Tissue Thromboplastin Inhibition test (TTI) in which PLs are extremely diluted. In fact, affinity-purified antibody preparations from 5 patients with antiphospholipid syndrome did not express or only weakly expressed anticoagulant activity in both tests; the mean ratios of coagulation times obtained with purified antibodies and that of control buffer were 1.11 and 1.0 for KCT and TTI, respectively. On the contrary, the mean ratios in dRVVT were 1.31 and 1.49 at a PLs dilution of 1:8 and 1:64, respectively. Therefore, the presence of LA activity due to autoantibodies to β2-GPI is characterized by a positive dRVVT and negative or only weakly positive KCT and TTI.
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References
- 1 Feinstein I D, Rapaport I. S. Acquired inhibitors of blood coagulation. Progress Haemost Thromb 1972; 1: 75-95.
- 2 Takeya H, Mori T, Gabazza EC, Kuroda K, Deguchi H, Matsuura E, Ichikawa K, Koike T, Suzuki K. Anti-β2-Glycoprotein I monoclonal antibodies with lupus anticoagulant activity enhance the β2-Glycoprotein I binding to phospholipids. J Clin Invest 1997; 99: 2260-8.
- 3 Arnout J, Wittevrongel C, Vanrusselt M, Hoylaerts M, Vermylen J. β2-Glycoprotein I dependent lupus anticoagulants form stable bivalent antibody-β2-Glycoprotein I complexes on phospholipid surfaces. Thromb Haemost 1998; 79: 79-86.
- 4 Willems GM, Janssen MP, Pelsers MMAL, Comfurius P, Galli M, Zwaal RFA, Bevers EM. Role of divalency in the high affinity binding of anti-cardiolipin antibody-β2-Glycoprotein I complexes to lipid membranes. Biochemistry 1996; 35: 13833-42.
- 5 Brandt JT, Triplett DA, Alvin B, Scharrer I. Criteria for the diagnosis of lupus anticoagulant. Thromb Haemost 1995; 74: 1185-90.
- 6 Galli M, Finazzi G., Bevers EM, Barbui T. Kaolin Clotting Time and dilute Russell’s Viper Venom Time distinguish between prothrombin-dependent and β2-Glycoprotein I-dependent antiphospholipid antibodies. Blood 1995; 86: 617-23.
- 7 Pengo V, Balestrieri G, Tincani A, Spatola L, Biasiolo A, Brocco T. Utilization of dilute Russell’s Viper Venom Time to detect autoantibodies against β2-Glycoprotein I which express anticoagulant activity in the presence but not in the absence of exogenous phospholipids. Thromb Haemost 1997; 77: 123-6.
- 8 Viard JP, Amoura Z, Bach JF. Association of anti-β2-glycoprotein I antibodies with lupuS-type circulating anticoagulant and thrombosis in systemic lupus erythematosus. Am J Med 1992; 93: 181-6.
- 9 Pengo V, Biasiolo A, Brocco T, Tonetto S, Ruffatti A. Autoantibodies to phospholipid binding plasma proteins in patients with thrombosis and phospholipid-reactive antibodies. Thomb Haemost 1996; 75: 721-4.
- 10 Thiagarajan P, Pengo V, Shapiro SS. The use of dilute Russell Viper Venom Time for the diagnosis of lupus anticoagulants. Blood 1986; 68: 869-73.
- 11 Biasiolo A, Pengo V. Antiphospholipid antibodies are not present in the membrane of gel-filtered platelets of patients with IgG anticardiolipin antibodies, lupus anticoagulant and thrombosis. Blood Coag Fibrinol 1993; 4: 425-8.
- 12 Balestrieri G, Tincani A, Spatola L, Allegri F, Prati E, Cattaneo R, Valesini G, Del Papa N, Meroni P. Anti-β2-Glycoprotein I antibodies: a marker of antiphospholipid syndrome?. Lupus 1995; 4: 122-30.
- 13 Pengo V, Biasiolo A, Fior MG. Autoimmune antiphospholipid antibodies are directed against a cryptic epitope expressed when β2-Glycoprotein-I is bound to a suitable surface. Thromb Haemost 1995; 73: 29-34.
- 14 Shapiro SS, Thiagarajan P. Lupus anticoagulants. Progr Hemostas Thromb 1982; 6: 263-85.
- 15 Exner T, Rickard A, Kronenberg H. A sensitive test demonstrating Lupus Anticoagulant and its behavioural patterns. Br J Haematol 1978; 40: 143-51.
- 16 Mann KG, Krishnaswamy S, Lawson JH. Surface-dependent hemostasis. Sem Haematol 1992; 29: 213-26.
- 17 Schleider MA, Nachman RL, Jaffe EA, Coleman M. A clinical study of the lupus anticoagulant. Blood 1976; 48: 499-509.
- 18 Shi W, Chang BH, Hogg J, Chesterman CN. Anticardiolipin antibodies block the inhibition by β2-Glycoprotein I of the factor Xa generating activity of platelets. Thromb Haemost 1993; 70: 342-5.