Intracellular Factor XIII Crosslinks Platelet Cytoskeletal Elements upon Platelet Activation

Katherine Serrano; Dana V. Devine

doi:10.1055/s-0037-1613204

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2002; 88(02): 315-320
DOI: 10.1055/s-0037-1613204

In Focus

Schattauer GmbH

Intracellular Factor XIII Crosslinks Platelet Cytoskeletal Elements upon Platelet Activation

Katherine Serrano

¹Departments of Biochemistry and Molecular Biology, Canada

³Departments of Canadian Blood Services, Vancouver, BC, Canada

,

Dana V. Devine

¹Departments of Biochemistry and Molecular Biology, Canada

²Departments of Pathology and Laboratory Medicine, University of British Columbia, Canada

³Departments of Canadian Blood Services, Vancouver, BC, Canada

› Author Affiliations

Abstract

Summary

The platelet cytoplasm contains approximately half of the factor XIII (FXIII) transglutaminase content circulating in blood, yet the function of cytoplasmic FXIII is poorly understood. This study investigated functions of platelet FXIII in internal platelet processes by studying the interactions of FXIII with platelet cytoskeletal proteins. FXIII was present in cytoskeletal fractions of platelet lysate separated by centrifugation. When cytoskeletal fractions were immobilized on nitrocellulose membranes, thrombin-activated rFXIII (rFXIIIa*) or calcium iontreated rFXIII (rFXIIIa°) bound to some of these proteins, whereas untreated rFXIII did not. Utilizing fluorescence microscopy, an actin polymerization-dependent transient translocation of FXIII from a diffuse homogeneous distribution throughout the cytoplasm to the platelet periphery was observed upon platelet activation, suggesting an association with cytoskeletal proteins. Transglutaminase activity increased in cytoskeletal fractions of activated versus non-activated platelets. Immunoblotting analysis of platelet cytoskeletal fractions identified filamin and vinculin as being crosslinked upon platelet activation.

Keywords

Platelet activation - factor XIII - filamin - vinculin - crosslinking

Full Text

References

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