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Thromb Haemost 2002; 88(01): 149-154
DOI: 10.1055/s-0037-1613168
Review Article
Schattauer GmbH

Recombinant Soluble P-Selectin Glycoprotein Ligand-Ig (rPSGL-Ig) Attenuates Infarct Size and Myeloperoxidase Activity in a Canine Model of Ischemia-Reperfusion

Kai Wang
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Xiaorong Zhou
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Zhongmin Zhou
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Khaldoun Tarakji
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Jian Xin Qin
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Marta Sitges
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Takahiro Shiota
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Farhad Forudi
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Robert G. Schaub
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Anjali Kumar
1   Wyeth/Genetics Institute, Andover, MA, USA
,
Marc S. Penn
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
Eric J. Topol
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
,
A. Michael Lincoff
1   Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH
› Author Affiliations
Further Information

Publication History

Received 19 December 2001

Accepted after resubmission 18 March 2002

Publication Date:
09 December 2017 (online)

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Summary

The role of P-selectin in the process of reperfusion injury was evaluated using a recombinant soluble P-selectin glycoprotein ligand-Ig (rPSGL-Ig) in a canine coronary artery balloon occlusion model. rPSGL-Ig (1 mg/kg) or saline was given as an intravenous bolus 15 min before balloon deflation. Balloon occlusion time was 90 min followed by either 120 min or 7 days reperfusion. Infarct size was significantly reduced in the treatment group when expressed either as percentage of the area at risk or as absolute infarct size. Histological analysis showed that extensive myocardial injury and neutrophil infiltration were reduced by rPSGL-Ig. Myeloperoxidase activity (MPO) was significantly reduced in the risk area in the rPSGL-Ig group. Left ventricular ejection fraction was significantly less impaired during the first 24 h after reperfusion in the rPSGL-Ig group, although there was no difference by 7-day follow-up. Thus, administration of rPSGL-Ig decreases myocardial injury and inflammatory response for at least 7 days after reperfusion of ischemic myocardium.

Keywords

P-selectin - rPSGL-Ig - inflammatory response - reperfusion injury
 

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