New and Effective Treatment of Experimentally Induced Venous Thrombosis with Anti-inflammatory rPSGL-Ig

Daniel Myers; Shirley Wrobleski; Frank Londy; Beverly Fex; Angela Hawley; Robert Schaub; Lazar Greenfield; Thomas Wakefield

doi:10.1055/s-0037-1613014

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2002; 87(03): 374-382
DOI: 10.1055/s-0037-1613014

In Focus

Schattauer GmbH

New and Effective Treatment of Experimentally Induced Venous Thrombosis with Anti-inflammatory rPSGL-Ig

Authors

Daniel Myers

¹The Unit for Lab ora tory Anim al Me dicine, Ann Arb or, MI
Shirley Wrobleski

⁴Jobst Vascular Laboratory, Section of Vascular Surgery, Department of Surgery
Frank Londy

²Department of Radiology, University of Michigan, Ann Arb or, MI
Beverly Fex

⁴Jobst Vascular Laboratory, Section of Vascular Surgery, Department of Surgery
Angela Hawley
Robert Schaub

³Genetics Institute, Wyeth Inc., And over, MA, USA
Lazar Greenfield

⁴Jobst Vascular Laboratory, Section of Vascular Surgery, Department of Surgery
Thomas Wakefield

⁴Jobst Vascular Laboratory, Section of Vascular Surgery, Department of Surgery

Abstract

Summary

Background

P-selectin antagonism decreases thrombosis and inflammation in animal models of venous thrombosis (VT) prophylaxis. This study defines results using a P-selectin receptor antagonist for VT treatment.

Methods

Eight juvenile baboons underwent 6 h of iliofemoral venous stasis to produce an occlusive VT. Two days later, animals were treated for 14 days with rPSGL-Ig, 4 mg/kg (n3), LMWH (n2) or saline (n3) and treatment continued weekly (rPSGL-Ig) or daily (LMWH, saline). The animals were examined and sacrificed 14 days after treatment initiation (n4) or on day 90 (n4).

Results

Percent spontaneous vein reopening revealed a significant increase (p <0.05) in the proximal iliac vein in rPSGL-Ig and LMWH animals compared to controls (62%, 70% vs 8%), without differences in inflammation. No anticoagulation, thrombocytopenia, or wound complications were found in rPSGL-Ig animals. At 90 days, recanalization with iliac vein valve competence was found in treated animals.

Conclusion

rPSGLIg successfully treated established VT without anticoagulation.

Keywords

Venous thrombosis - rPSGL-Ig - P-selectin - inflammation - recanalization

Full Text

References

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