Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608591
Lecture Session – Phytopharmacology/Extract Pharmacology III
Georg Thieme Verlag KG Stuttgart · New York

Effects of Inonotus hispidus extracts and compounds on human immunocompetent cells

C Gründemann
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
M Arnhold
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
S Meier
3   Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany, Greifswald, Germany
,
C Bäcker
3   Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany, Greifswald, Germany
,
M Garcia-Käufer
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
F Grunewald
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
C Steinborn
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
M Klemd Amy
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
R Wille
2   ABNOBA GmbH, Pforzheim, Germany, Pforzheim, Germany
,
R Huber
1   Center for Complementary Medicine, Institute for Environmental Health Sciences and Hospital Infection Control, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany
,
U Lindequist
3   Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany, Greifswald, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Inonotus hispidus is used as a traditional medicine in China. Previous investigations revealed promising immunomodulatory activity of fruit body extracts of I. hispidus. Bioactivity-guided fractionation showed that hispolon and hispidin were active substances.

In this study, we analysed effects of I. hispidus extract (IHE) and selected constituents on different types of human immune cells and investigated the potential of IHE as a medicinal mushroom. The influence of IHE on activity and maturation of human T cells, purified NK cells and dendritic cells (DCs) was analysed using flow cytometric-based surface marker expression. The cell-division characteristics of the activated T cells were assessed by membrane-permeable dye, and the function of NK cells was investigated by degranulation-CD107a assay. Apoptosis induction was assessed by surface staining of phosphatidylserine, and camptothecin (CPT) and cyclosporine A (CsA) were used individually as controls. Phytochemical analysis, using TLC chromatograms and HPLC analysis, was conducted to characterize the IHE.

IHE increased the activation and diminished the proliferation of activated human T cells in the presence of apoptosis. NK cell activity and function were dose-dependently increased. Surface marker expression of DCs demonstrated that IHE has the potential to induce maturation. TLC and HPLC analyses showed that the extract contained hispidin and hispolon. Investigations using hispidin and hispolon demonstrated similar, albeit non-congruent results, with extracts on measured parameters.

The results indicate that extracts from I. hispidus and their constituents, hispidin and hispolon, interfere with function of multiple immune cells, thus providing a rationale for their potential as a medicinal mushroom.