Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608575
Lecture Session – Miscellaneous
Georg Thieme Verlag KG Stuttgart · New York

Quantification of antigenotoxic flavonoids in the stem bark of Erythrina latissima by an HPLC-UV method

Y Zarev
1   Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
3   Department of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, Sofia, Bulgaria
,
K Foubert
1   Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
,
T Naessens
1   Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
,
I Ionkova
3   Department of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, Sofia, Bulgaria
,
E Elgorashi
4   Toxicology and Ethnoveterinary Medicine; Food, Feed and Veterinary Public Health, ARC-Ondertsepoort Veterinary Institute, Onderstepoort, South Africa
,
L Verschaeve
2   Toxicology, Scientific Institute of Public Health, Brussels, Belgium
,
L Pieters
1   Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
,
S Apers
1   Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Erythrina latissima E. Mey (Leguminosae) is a tree up to 24 m tall and generally found in Southern Africa. The stem bark shows high potential for livestock industry since dietary supplementation might induce antimutagenic activity and subsequently decrease animal diseases and increase production of eggs, milk and meat [1 – 3]. Five new and 18 known flavonoids were identified in the plant material and evaluation of their antigenotoxic properties against aflatoxin B1 induced genotoxicity revealed most flavonoids were active. Sigmoidin A and B showed an activity (IC50= 18.7 µg/mL) equivalent to that of curcumin (IC50= 18.4 µg/mL) [1]. Industrial use of E. latissima includes the necessity of the development and validation of a method for quantification of total flavonoids in the stem bark of E. latissima. The sample extraction method and the HPLC-UV conditions were optimized. The analytical validation was performed according to ICH guidelines. The calibration model was linear for both rutin and naringenin, used as a secondary standard for flavonoid glycosides and aglycons, respectively. Statistical analysis showed that for both glycosides and aglycons the method seems precise on different days and concentration levels. By mean of a recovery experiment, the method showed to be accurate concerning aglycons. This method is fully applicable for the quantification of non-glycosylated flavonoids of the stem bark of E. latissima (average amount 1.44%) and indicative for the amount the glycosylated flavonoids (average amount 1.97%). Ongoing In vivo experiments with the quantified extract of E. latissima will provide additional information on its use as a chemopreventive agent.

[1] Zarev Y; Foubert K, de Almeida VL, Anthonissen R, Elgorashi E, Apers S, Ionkova I, Verschaeve L, Pieters L, Pytochemistry, submitted.

[2] Binder EM, Tan LM, Chin LJ, Handl J, Richard J, Anim Feed Sci Technol 2007; 137: 265 – 282.

[3] Pier AC, J Anim Sci 1992; 70: 3964 – 3967.