Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608550
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Association of Pterodon pubescens and Cordia verbenacea in inflammation and thermal nociception models

R Tarkany Basting
1   Postgraduate Program in Medical Sciences – Faculty of Medical Science – FCM/State University of Campinas – Unicamp, Campinas, Brazil
2   CPQBA, State University of Campinas – Unicamp, Campinas, Brazil
,
I Maria de Oliveira Sousa
1   Postgraduate Program in Medical Sciences – Faculty of Medical Science – FCM/State University of Campinas – Unicamp, Campinas, Brazil
2   CPQBA, State University of Campinas – Unicamp, Campinas, Brazil
,
H Moreira Spindola
2   CPQBA, State University of Campinas – Unicamp, Campinas, Brazil
,
E de Carvalho João
3   Faculty of Pharmaceutical Sciences – FCF/State University of Campinas – Unicamp, Campinas, Brazil
,
A Foglio Mary
3   Faculty of Pharmaceutical Sciences – FCF/State University of Campinas – Unicamp, Campinas, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Pterodon pubescens (Pp) and Cordia verbenacea (Cv) species are traditionally used in Brazilian folk medicine for the known anti-inflammatory effects [1]. The purpose of this study was to assess the synergistic effect of the dichloromethane extract from the fruits of Pp with the essential oil from leaves of Cv in paw edema induced by carrageenan, hot plate and tail flick models. All animals (male Swiss mice n = 6) were orally treated with the association of the extracts based on their ED50, corresponding to: A50 (32.5 mg/kg of Pp + 82.5 mg/kg of Cv); A100 (65 mg/kg of Pp + 165 mg/kg of Cv) and A200 (130 mg/kg of Pp + 330 mg/kg of Cv). All experimental animal procedures were approved by Ethics Committee n° 3181 – 1. In hot plate and tail flick models, all associations tested did not show significant efficacy against nociception due to thermal stimulation. In paw edema induced by carrageenan, the 3 associations tested showed a significant reduction of edema from the fourth hour after carrageenan administration, the activity remaining after 48 hours. From the fourth hour of evaluation, A200 showed 26% inhibition in the volume of edema when compared to the vehicle (negative control). After 6 hours of edema induction, A50 and A100 doses, respectively, showed inhibition of edema in 19% and 23%, while A200 showed reduction of approximately 39% when compared to vehicle. After 24 and 48 hours of edema induction, all associations showed reduction of approximately 32% when compared to vehicle. The results demonstrated that the associations showed a significant anti-edematogenic activity, but did not show significant results in thermal stimulus models. Therefore this extracts association may represent a potential therapeutic advantage for the clinical treatment of inflammation.

[1] Dutra, R.C. et al. Pharmacol Res 2016; 112, 4 – 29.