Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608548
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Phytochemical profiles and antioxidant activity of Baccharis dracunculifolia and Baccharis trimera ethanolic extracts

B Marques
1   CITAB-Centre for the Research and Technology of Agro-Environmental and Biological Sciences, Department of Biology, University of Minho, Campus de Gualtar, 4710 – 057 Braga, Portugal, Braga, Portugal
,
M Silvana Alves
2   Faculdade de Farmácia e Bioquímica, Departamento Farmacêutico, Universidade Federal de Juiz de Fora, Juiz de Fora, MG – Brasil, Juiz de Fora, Brazil
,
A Silva Filho
2   Faculdade de Farmácia e Bioquímica, Departamento Farmacêutico, Universidade Federal de Juiz de Fora, Juiz de Fora, MG – Brasil, Juiz de Fora, Brazil
,
A Dias
1   CITAB-Centre for the Research and Technology of Agro-Environmental and Biological Sciences, Department of Biology, University of Minho, Campus de Gualtar, 4710 – 057 Braga, Portugal, Braga, Portugal
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
24. Oktober 2017 (online)

 

Baccharis sp. plants have been demonstrated antioxidant, neuroprotective and antiinflammatory properties. The goals of this work are to i- characterize the phytochemical profile of Baccharis dracunculifolia (BD) and Baccharis trimera (BT) extracts, and ii- evaluate their in vitro antioxidant activity.

Phytochemical profiles of BD and BT ethanolic extracts were obtained by HPLC-DAD. The phytochemical analysis showed that both plants are mainly composed by phenolic compounds (flavonoids and phenolic acids derivatives), which are known to have antioxidant properties. Antioxidant activity was evaluated using several in vitro models: free radical scavenging (DPPH method), iron quelation (ferrozine method), NO and SO scavenging, reduction power (FRAP method), and lipid peroxidation (β-carotene bleaching assay); quercetin was used as a reference antioxidant standard. Both extracts showed antioxidant activity in all assays, except BT that did not display an efficient chelating iron activity. In general, BD exhibited better antioxidant activities (EC50 mg dw/ml; DPPH: 54.3 ± 4.9; SO: 11.1 ± 2.2; β-carotene: 60,0 ± 5.3) than BT. BD showed higher activities in NO scavenging (EC50, 217.1 ± 19.5) and FRAP assays (EC50, 35.0 ± 3.0). The antioxidant activities of extracts were significantly different in all assays (p< 0.05), except for DPPH and FRAP assays. Remarkably, BD demonstrated significant higher activity in scavenging SO radical than quercetin, and both plants displayed better activity than quercetin in reducing ferric ions. Thus, our studies proved that BD and BT can be used as source of compounds with pharmacological properties, namely antioxidants, which could be helpful in neuroprotection and other oxidative stress-related diseases.

This work was supported by Fundação para a Ciência e Tecnologia (FCT), project PEst-OE/AGR/UI4033/2014, INTERACT project -NORTE-01 – 0145-FEDER-000017, HEALTHKAKI, N° 010672, Programa Operacional Regional do Norte, Portugal 2020