Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608514
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Distribution of secoiridoid glycosides in the root system of the medicinal plant Gentiana lutea L. subsp. aurantiaca

Ó González-López
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
S Mayo
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
Á Rodríguez-González
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
G Carro-Huerga
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
V Suárez Villanueva
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
T Berninger
2  AIT Austrian Institute of Technology GmbH, Center for Health & Bioresources.Muthgasse 11, 1190, Vienna, Spain
,
PA Casquero
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Gentiana lutea L. subsp. aurantiaca M. Lainz is an endemic medicinal plant from the Iberian Peninsula. Easily recognizable by the orange/reddish colour of its flowers, several other differences as regards habitat, genetics and concentration in bitter compounds have been reported when comparing subspecies aurantiaca with other G. lutea subspecies.

The present study evaluates the variability in the concentration of the main bitter compounds (secoiridoid glycosides): amarogentin, gentiopicroside, sweroside and swertiamarin, between the cortex and vascular tissues of gentian roots. Methanolic extracts from both tissues obtained from complete root systems of G. lutea subsp. aurantiaca wild collected in the Cantabrian Mountains (Spain) have been analysed by HPLC chromatography.

Obtained results show how the concentration of all analysed compounds is significantly higher in the cortex than in the vascular tissues, but as it can be observed in the Table 1, the difference is much higher as regards the amarogentin. As known, with age the relative amounts of cortex and vascular tissues vary, and with it also the concentration of analysed compounds in the roots. These results underline the necessity of quality control measurements to ensure products with homogeneous characteristics and also suggest that the selection of optimal roots would increase the yield in secoiridoid glycosides.

Tab. 1: Secoiridoid glycosides (mg g of root dry weight) in the cortex/vascular tissues in the roots of wild collected G. lutea L. subsp. aurantiaca. Different letters for every bitter compound are significantly different (p ≤0.05) according to Student's T test.

Cortex

mg/g

Vascular tissues

mg/g

Variation

%

Gentiopicroside

7.187 a

5.381 b

33.6

Amarogentin

0.088 a

0.024 b

261.5

Sweroside

0.174 a

0.108 b

61.7

Swertiamarin

0.370 a

0.309 b

19.8

[1] Gonzalez-Lopez O. Polanco C. Gyorgy Z. Pedryc A. Casquero PA. Journal of Molecular Sciences 2014: 10052 – 10066

[2] Gonzalez-Lopez O. Carro G. Aiello N. Scartezzini F. Casquero PA. Planta Medica 2014; 80: 1489 – 1490