Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608459
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Control of Xylotrechus arvicola (Coleoptera: Cerambycidae) larvae population by inoculating Trichoderma spp. in vine wood

Á Rodríguez-González
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
V Suárez Villanueva
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
S Mayo
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
G Carro-Huerga
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
Ó González-López
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
S Gutiérrez
2  Area of Microbiology, University School of Agricultural Engineers, Universidad de León, Ponferrada Campus, Av. Astorga s/n, 24401, Ponferrada, Spain
,
H Peláez
3  Freelance, Vicente Aleixandre 24, 47008, Valladolid, Spain
,
PA Casquero
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Xylotrechus arvicola (Coleoptera: Cerambycidae) is a grapevine (Vitis vinifera) pest in the Iberian Peninsula causing the spread of wood diseases. Trichoderma (Teleomorph: Hypocrea) is a fast growing opportunistic fungus, which produces antimicrobial compounds1. Moreover, it can be used as biological control of pests2. This study's aim is to evaluate Trichodema spp. efficacy on controlling X. arvicola larvae, simulating field conditions. One millilitre of spore suspension (1 × 107esp/ml) of Trichoderma spp. was applied directly to vine with a manual diffuser. One hour after, four X. arvicola larvae were placed on the vine. Vines were opened 135 days later to count larvae and extract wood samples. Four samples per treatment from rhytidome and galleries per vine in four replicates were incubated on Petri dishes with Rose Bengal-Chloramphenicol Agar medium at controlled conditions during 7 days.

Tab. 1: X. arvicola dead larvae with Trichoderma spp., number of isolations and percentage of isolates showing growth of Trichoderma spp. from vine wood (rhytidome and galleries)

Trichoderma

treatment

(Isolated

number)

Mortality

(%) of

X. arvicola

larvae

Rhytidome

isolations

Rhytidome

(%) with

Trichoderma

Galleries

isolations

Galleries (%)

with

Trichoderma

T. harzianum

(T-019)

81.25 ± 6.25A

21

76.19

16

75.00

T. gamsii

(T-066)

68.75 ± 23.66A

17

29.41

8

100.00

T. gamsii

(T-067)

31.25 ± 11.96B

16

37.50

12

33.33

T. gamsii

(T-070)

75.00 ± 10.21A

11

54.54

4

100.00

T. gamsii

(T-071)

81.25 ± 11.97A

12

33.33

11

45.45

Control

6.25 ± 6.25B

9

0.00

6

0.00

Different capital letters indicate significant differences among fungal isolates of dead larvae with Trichoderma spp. (Fisher's LSD. p < 0.05).

100% of T. gamsii (T-066) and T. gamsii (T-070) galleries isolates showed Trichoderma growth, confirming the ability of this fungus to move through the vine wood by the galleries created by X. arvicola larvae. T. gamsii (T-071) and T. harzianum (T-019) treatments showed the highest control over insect larvae with a 81.25% mortality. These results indicate Trichoderma should be considered for further studies to evaluate its efficacy to control X. arvicola on field conditions.

[1] Mayo S, Gutiérrez S, Malmierca MG, Lorenzana A, Campelo MP, Hermosa R, Casquero PA. Front. Plant Sci 2015, 6, 685.

[2] Abdul-Wahid, OA, Elbanna, SM. Afr. J. Microbiol. Res. 2012, 6: 1024 – 1032.