Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608360
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

In vitro effects of Trichoderma secondary metabolites on Phaeoacremonium aleophilum

G Carro-Huerga
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
S Mayo
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
Á Rodríguez-González
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
V Suárez Villanueva
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
Ó González-López
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
,
S Gutiérrez
2  Research Group of Engineering and Sustainable Agriculture, Area of Microbiology, University School of Agricultural Engineers, Universidad de León, Ponferrada, Spain
,
PA Casquero
1  Research Group of Engineering and Sustainable Agriculture, Research Institute of Environment, Natural Resources and Biodiversity, Universidad de León, Leon, Spain
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Trichoderma species have an important relevance as biocontrol or biological agents inhibiting phytopathogenic fungi growth. Some of the secondary metabolites Trichoderma produces have shown antifungal activity. Phaeoacremonium aleophilum has been described as one of the first fungi colonizers which cause the Grapevine Trunk Disease.

The aim of this study is to evaluate the efficiency of an array of trichoderma species mainly isolated from old vineyards in controlling this important grapevine wood pathogen.

This in vitro antifungal experiment was carried out using a celophane membrane over PDA growth medium. The membrane allows secondary metabolites to pass through whereas fungi mycelium is retained1.

Results show (Figure 1) T100 to be the strain with the highest inhibitory capacity percentage, being well over 50%. The following group of strains in the ranking, T077, T075, T084, T115, T127, T120, T080 and T074 exhibit a capacity between 30 and 40%. Finally, the strains coming after this group continue in a decreasing succession reaching an inhibitory capacity less than 10%.

Zoom Image
Fig. 1: Isolated Trichoderma strains with inhibitory capacity against P. aleophilum. Error bars are depicted. Different letters show significant differences. (Fisher's LSD. p < 0.05).

Pathogen growth is decreased by trichoderma secondary metabolites, stronly depending on trichoderma strain. Further trials are needed to identify the most efficient trichoderma strains at controlling Phaeoacremonium aleophilum.

[1]. Mayo, S, S. Gutiérrez, M.G. Malmierca, A. Lorenzana, M.P. Campelo, R. Hermosa, and P.A. Casquero. 2015. Front. Plant Sci. 6: 1 – 11.